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EC number: 212-773-3 | CAS number: 868-18-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- / 2-AA used as sole indicator of S9 mix efficacy; duplicate plating without scientific justification; only 1 plate for some positive controls; no result of 2-AA (+S9 mix) in 1 exp (TA 1537, TA 1538 and WP2 uvr A); AF-2 used as positive control of WP2 uvr A
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Microbial mutagenesis testing of substances compound report: F76-019, Potassium sodium tartrate
- Author:
- Simmon, V.F. and Eckford, S.L.
- Year:
- 1 979
- Bibliographic source:
- U.S. Departement of Commerce, National Technical Information Service, Springfield, U.S.A
- Reference Type:
- secondary source
- Title:
- Bacterial mutagenicity testing of 49 food ingredients givesvery few positive results
- Author:
- Prival, M.J. et al.
- Year:
- 1 991
- Bibliographic source:
- Mutation Research 260: 321-329
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- 21 July 1997
- Principles of method if other than guideline:
- - Principle of test: similar to OECD 471 but with several differences:
2-AA was used as sole indicator of S9 mix efficacy; duplicate plating without scientific justification; only 1 plate for some positive controls; no result of 2-AA (+S9 mix) in 1 exp (TA 1537, TA 1538 and WP2 uvr A); AF-2 used as positive control of WP2 uvr A - GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Potassium sodium tartrate
- EC Number:
- 206-156-8
- EC Name:
- Potassium sodium tartrate
- Cas Number:
- 304-59-6
- Molecular formula:
- C4H6O6.K.Na
- IUPAC Name:
- potassium sodium tartarate
- Test material form:
- solid
Constituent 1
Method
- Target gene:
- his operon, trp operon
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- S. typhimurium TA 1538
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- cofactor supplemented post-mitochondrial fraction (S9 mix), prepared from the livers of male rats, treated with Aroclor 1254 (500 mg/kg bw)
- Test concentrations with justification for top dose:
- Based on a range-finding study (performed in tester strain TA 100; doses applied: 0.3 - 10000 μg/plate), the following concentrations were used in the main experiments:
TA 1535 / TA 100:
1st exp.: 33.3, 100, 333.3, 1000, 3333.3 and 10000 µg/plate with and without metabolic activation
2nd exp.: 0.3, 3.3, 33.3, 100, 333.3, 1000, 3333.3 and 10000 µg/plate with and without metabolic activation
TA 1537 / TA 1538 / TA 98 / WP2 uvr A:
1st exp.: 0.3, 3.3, 33.3, 100, 333.3, 1000, 3333.3 and 10000 µg/plate with and without metabolic activation
2nd exp.: 33.3, 100, 333.3, 1000, 3333.3 and 10000 µg/plate with and without metabolic activation - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: potassium phosphate buffer
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- potassium phosphate buffer (0.067 M, pH 7)
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- 2-nitrofluorene
- sodium azide
- other: AF-2 (2-(2-Furyl)-3-(5-nitro-2-furyl) acrylamide), 2AA (2-Aminoanthracene)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in medium; in agar (plate incorporation) (Range Finding Test, first and second experiment)
DURATION
- Exposure duration: 48 h
NUMBER OF REPLICATIONS: 2
DETERMINATION OF CYTOTOXICITY
- Method: not provided - Statistics:
- Mean values were calculated.
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Additional information on results:
- RANGE-FINDING/SCREENING STUDIES: A range finding assay was performed in TA 100. No mutagenicity and toxicity were observed.
Any other information on results incl. tables
Table 1: Summary of test results (Exp. 1, Plate Incorporation Method)
With or without S9-Mix |
Test substance concentration (μg/plate) |
Mean number of revertant colonies per plate (average of 2 plates) |
|||||
Frameshift type |
Base-pair substitution type |
||||||
TA98 |
TA1537 |
TA1538 |
TA100 |
TA1535 |
WP2 uvrA |
||
– |
Solvent control (potassium phosphate buffer) |
24* |
16* |
13* |
204* |
41* |
50* |
0.3 |
22 |
13 |
15 |
- |
- |
49 |
|
3.3 |
23 |
15 |
13 |
- |
- |
51 |
|
33.3 |
21 |
18 |
19 |
204 |
49 |
47 |
|
100 |
24 |
12 |
10 |
198 |
43 |
41 |
|
333.3 |
22 |
14 |
25 |
201 |
38 |
45 |
|
1000 |
22 |
11 |
11 |
174 |
48 |
36 |
|
3333.3 |
28 |
19 |
14 |
205 |
47 |
48 |
|
10000 |
14 |
14 |
16 |
183 |
37 |
53 |
|
Positive controls (µg/plate) |
2-NF (5) |
9AA (50) |
2-NF (5) |
SA (0.5) |
SA (0.5) |
AF2 (0.1) |
|
Mean No. of colonies/plate |
359 |
185 |
692 |
411** |
264** |
103 |
|
Positive controls (µg/plate) |
2AA |
2AA |
2AA |
2AA |
2AA |
2AA |
|
Mean No. of colonies/plate |
39** |
nt |
nt |
181** |
37** |
72** |
|
+ |
Solvent control (potassium phosphate buffer) |
36* |
11* |
22* |
252* |
41* |
102* |
0.3 |
47 |
14 |
21 |
- |
- |
47 |
|
3.3 |
34 |
18 |
17 |
- |
- |
53 |
|
33.3 |
40 |
16 |
32 |
244 |
69 |
48 |
|
100 |
31 |
18 |
32 |
219 |
78 |
51 |
|
333.3 |
38 |
14 |
27 |
246 |
67 |
52 |
|
1000 |
45 |
12 |
25 |
259 |
59 |
59 |
|
3333.3 |
34 |
13 |
24 |
256 |
62 |
49 |
|
10000 |
35 |
19 |
34 |
239 |
52 |
54 |
|
Positive controls (µg/plate) |
2AA |
2AA |
2AA |
2AA |
2AA |
2AA |
|
Mean No. of colonies/plate |
965** |
nt |
nt |
360** |
119** |
166** |
2AA = 2-aminoanthracene
2-NF = 2-nitrofluorene
9AA = 9-aminoacridine
AF-2 = 2-(2-Furyl)-3-(5-nitro-2-furyl) acrylamide
SA = sodium azide
* = 4 results
** = 1 result
Table 2: Summary of test results (Exp. 2, Plate Incorporation Method)
With or without S9-Mix |
Test substance concentration (μg/plate) |
Mean number of revertant colonies per plate (average of 2 plates) |
|||||
Frameshift type |
Base-pair substitution type |
||||||
TA98 |
TA1537 |
TA1538 |
TA100 |
TA1535 |
WP2 uvrA |
||
– |
Solvent control (potassium phosphate buffer) |
15* |
5* |
11* |
77* |
15* |
33* |
0.3 |
- |
- |
- |
77 |
14 |
- |
|
3.3 |
- |
- |
- |
76 |
17 |
- |
|
33.3 |
21 |
5 |
14 |
78** |
14 |
35 |
|
100 |
20 |
4 |
9 |
70 |
11 |
36 |
|
333.3 |
16 |
5 |
18 |
77 |
14 |
41 |
|
1000 |
21 |
5 |
11 |
70 |
11 |
34 |
|
3333.3 |
17 |
3 |
15 |
82 |
16 |
36 |
|
10000 |
15 |
5 |
17 |
67 |
12 |
39 |
|
Positive controls (µg/plate) |
2-NF (5) |
9AA (50) |
2-NF (5) |
SA (0.5) |
SA (0.5) |
AF2 (0.1) |
|
Mean No. of colonies/plate |
359** |
154** |
591** |
417 |
300 |
1581 |
|
Positive controls (µg/plate) |
2AA |
2AA |
2AA |
2AA |
2AA |
2AA |
|
Mean No. of colonies/plate |
18** |
3** |
19** |
89** |
7** |
nt |
|
+ |
Solvent control (potassium phosphate buffer) |
27* |
7* |
21* |
85* |
5* |
40* |
0.3 |
- |
- |
- |
65 |
9 |
- |
|
3.3 |
- |
- |
- |
84 |
7 |
- |
|
33.3 |
44 |
9 |
26 |
75 |
7 |
44 |
|
100 |
36 |
9 |
25 |
66 |
6 |
58 |
|
333.3 |
27 |
8 |
25 |
84 |
6 |
43 |
|
1000 |
28 |
8 |
24 |
71 |
5 |
52 |
|
3333.3 |
29 |
5 |
25 |
72 |
6 |
49 |
|
10000 |
27 |
6 |
21 |
65 |
7 |
57 |
|
Positive controls (µg/plate) |
2AA |
2AA |
2AA |
2AA |
2AA |
2AA |
|
Mean No. of colonies/plate |
104** |
41** |
89** |
882** |
76** |
nt |
2AA = 2-aminoanthracene
2-NF = 2-nitrofluorene
9AA = 9-aminoacridine
AF-2 = 2-(2-Furyl)-3-(5-nitro-2-furyl) acrylamide
SA = sodium azide
* = 4 results
** = 1 result
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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