Registration Dossier
Registration Dossier
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EC number: 203-981-5 | CAS number: 112-52-7
Toxicity to microorganisms
Administrative data
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- 1986
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
- Remarks:
- The report is not sufficiently documented. Details on study performance are missing. Hence the report cannot be used for assessment.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 986
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: determination of bacterial toxicity with Pseudomonas putida according to Bringman and Kühn
- Version / remarks:
- not specified
- Deviations:
- not specified
- Principles of method if other than guideline:
- Method: other: Bringmann-Kühn, Inhibition of cell growth of Pseudomonas putida
- GLP compliance:
- no
Test material
- Reference substance name:
- 1-chlorododecane
- EC Number:
- 203-981-5
- EC Name:
- 1-chlorododecane
- Cas Number:
- 112-52-7
- Molecular formula:
- C12H25Cl
- IUPAC Name:
- 1-chlorododecane
- Test material form:
- liquid
- Details on test material:
- Test substance: as prescribed by 1.1 - 1.4
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The test substance is directly added to the solution of bacteria.
Test organisms
- Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- Solution with bacteria
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- pH:
- 7.0-7.5
- Nominal and measured concentrations:
- concentrations of 50 mL/L, 25 mL/L, 10 mL/L, and 5 mL/L
- Details on test conditions:
- Type: aquatic
A stock solution has been prepared and has been diluted 1:2, 1:4, and 1:8. Corresponding to 50, 25,10, and 5 mL/L test substance concentrations.
The test substance solution has been directly added to the bacteria. The absorbance of the bacteria solution has been determined after 48 h incubation.
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Effect conc.:
- 33 g/L
- Nominal / measured:
- nominal
- Basis for effect:
- growth inhibition
- Details on results:
- The test substance started to inhibit the cell growth of Pseudomonas putida at a concentration of 38 mL/L, corresponding to 33 g/L.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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