Reaction products of pentaerythritol, propoxylated and 1-chloro-2,3-epoxypropane with hydrogen sulfide

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

December 1969

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

The study meets the EC Standards (conducted equivalent to OECD 401). Non GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1969

Report date:

1969

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

yes

Remarks:

limited study design details in the report, no details on environmental conditions: not expected to affect the outcome of the study

GLP compliance:

no

Remarks:

not present at the time of performance.

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Reaction products of pentaerythritol, propoxylated and 1-chloro-2,3-epoxypropane with hydrogen sulfide

EC Number:

701-196-7

Cas Number:

72244-98-5

Molecular formula:

not applicable

IUPAC Name:

Reaction products of pentaerythritol, propoxylated and 1-chloro-2,3-epoxypropane with hydrogen sulfide

Test material form:

other: liquid

Details on test material:

- Name of test material (as cited in study report): DION® Polymercaptan Resin DPM 3-800LC
- Description: viscous, amber liquid

Test animals

Species:

rat

Strain:

other: Charles River strain (COBS)

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Charles River Breeding Laboratories, Inc. , North Wilmington, Massachusetts
- Age at study initiation: no data
- Weight at study initiation: 150 to 198 g
- Fasting period before study: 16 hours
- Housing: Housed in stock cages. Following oral administration of the test material, the rats were housed individually in observation cages.
- Diet: free access to standard laboratory rat diet (Purina Rat Chow)
- Water: free access to water
- Acclimation period: 5 days

ENVIRONMENTAL CONDITIONS
No data

Administration / exposure

Route of administration:

other: oral, using a hypodermic syringe equipped with a ballpointed intubating needle.

Vehicle:

unchanged (no vehicle)

Details on oral exposure:

Rats were intubated with previously calculated doses of the undiluted test material.

Doses:

1400, 2000, 3000 and 4600 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Body weights: At day 0 and day 14. Mortalities and/or reactions observed were recorded during the observation period of 14 days.
- Necropsy of survivors performed: yes

Statistics:

LD50 calculated using the techniques of Weil, Thompson, and Thompson and Weil.

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LD50

Effect level:

2 600 mg/kg bw

Based on:

test mat.

Mortality:

1400 mg/kg: no mortality
2000 mg/kg: 3 animals died
3000 mg/kg: 6 animals died (3 males, 3 females)
4600 mg/kg: all animals were found dead

Clinical signs:

other: Hyperpnea, hypoactivity, ruffed fur, tremors, dyspnea, muscular weakness, clonic convulsions (intermittent) and emaciation (highest dose only) were observed during the observation period.

Gross pathology:

No abnormalities were observed.

Applicant's summary and conclusion

Interpretation of results:

other: not classified according to Regulation (EC) No 1272/2008

Remarks:

Criteria used for interpretation of results: EU

Conclusions:

In an acute oral toxicity study with DPM 3-800LC with male and female rats, performed equivalent to OECD 401 guideline, an LD50 of 2600 mg/kg bw was calculated.

Executive summary:

The test substance DPM 3 -800LC was tested in an acute oral toxicity study with male and female rats, performed equivalent to OECD 401 guideline. Deaths occured at the 2000, 3000 and 4600 mg/kg dose levels. Hyperpnea, hypoactivity, ruffed fur, tremors, dyspnea, muscular weakness, clonic convulsions (intermittent) and emaciation were observed during the observation period. No effect on body weight was observed.

Based on the results, an LD50 of 2600 mg/kg bodyweight was calculated.