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EC number: 257-297-7 | CAS number: 51581-32-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1999-04-06 to 1999-06-07
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- Adopted 21st July 1997
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 3-pyridyl dimethylcarbamate
- EC Number:
- 257-297-7
- EC Name:
- 3-pyridyl dimethylcarbamate
- Cas Number:
- 51581-32-9
- Molecular formula:
- C8H10N2O2
- IUPAC Name:
- pyridin-3-yl N,N-dimethylcarbamate
Constituent 1
- Specific details on test material used for the study:
- - CAS No.: 51581-32-9
- Batch No.: TMFP 304
- Purity: at least 99 %
- Solubility in water: Soluble
- Date of expiry: December 1999
- Storage: In the refrigerator, in the dark
- Appearance: colorless liquid
- pH: 7.92
Method
- Target gene:
- Histidine
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: TA97a, TA98, TA100, TA102 and TA1535
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254 rat homogenised liver (S9) activation system
- Test concentrations with justification for top dose:
- Test substance: 5000, 1667, 556, 185 and 62 µg / plate
The concentrations for the first experiment were set according to the preliminary toxicity test. At 5000 µg / plate, a reduction of the bacterial background was seen but the revertant colonies were normal and could be counted. 5000 µg / plate is the highest recommended concentration recommended for the Ames test. - Vehicle / solvent:
- Vehicle used: None
Controlsopen allclose all
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: 4-Nitro-o-phenylene-diamine
- Remarks:
- -S9, TA97a, 10 µg / plate
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- Remarks:
- -S9, TA98, 2 µg / plate
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- -S9, TA100, 2µg / plate ; TA1535, 1 µg / plate
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: t-Butyl-hydroperoxide
- Remarks:
- -S9, TA102, 50 µg / plate
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- 7,12-dimethylbenzanthracene
- Remarks:
- +S9, TA97a, 5 µg / plate
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: 2-Aminoanthracene
- Remarks:
- +S9, TA98, 1 µg / plate ; TA100, TA1535, 2 µg / plate
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: 1,8 - Dihydroxy-anthraquinone
- Remarks:
- +S9, TA102, 50 µg / plate
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: Plate incorporation assay in which bacteria, test substance (and microsomes) are in contact on the plate without preceding incubation in the liquid state. The number of viable cells in the overnight-cultures is in the range of 2 X 10^8 cells per ml.
For each sample the following solutions were combined:
- 0.1 mL of the overnight culture of the bacteria
- 0.5 mL of S9-mix (or phosphate buffered saline for samples without metabolic activation)
- 0.1 mL of the appropriate test- or reference substance solution and
- 2 mL of top agar
The combined solutions were mixed and spread over a plate with minimal agar (9 cm diameter). After the top agar had solidified, the plates were incubated at 37 °C until the colonies were visible (2 days). - Rationale for test conditions:
- No specified
- Evaluation criteria:
- The criteria for a positive result are:
A reproducible increase of the number of revertants to more than the following threshold values for at least one of the concentrations:
- For the strains with a low spontaneous revertant rate i.e. TA98 and TA1535: The twofold of the amount of the spontaneous revertants
- For the strains with a high spontaneous revertant rate i.e. TA97a, TA100 and TA102: The 1 2/3 fold of the amount of the spontaneous revertants.
These threshold values were derived from the variations in the control samples of our historic data of the Ames test.
Results and discussion
Test results
- Key result
- Species / strain:
- S. typhimurium, other: TA97a, TA98, TA100, TA102 and TA1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- - Positive control substances: All positive control substances increased the mutation frequency to more than the threshold
- Solubility: The test substance was sufficiently soluble in water, no precipitation of the test substance was visible in any stage of the experiments
Applicant's summary and conclusion
- Conclusions:
- In this study, the test item was classified as non-mutagenic in an Ames test according to OECD 471 under the UN GHS Criteria.
- Executive summary:
In a reverse bacterial mutation test, conducted according to OECD guideline 471, strains of Salmonella typhimurium TA97a, TA98, TA100, TA102 and TA1535 were exposed to the test item dissolved in water at concentrations ranging from 62 to 5000 µg / plate according to the direct plate incorporation method in the presence and absence of an external S9 metabolising system. All positive control groups showed significantly increased mutation frequencies which demonstrate the sensitivity of the test system. No increase in the mutation frequency to more than the threshold values was obtained in any of the concentrations tested. Based on these results, the test item is considered non-mutagenic according to the UN GHS Criteria.
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