Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 257-297-7 | CAS number: 51581-32-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1999-02-23 to 1999-07-09
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Version / remarks:
- Adopted, 12 February 1999
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 3-pyridyl dimethylcarbamate
- EC Number:
- 257-297-7
- EC Name:
- 3-pyridyl dimethylcarbamate
- Cas Number:
- 51581-32-9
- Molecular formula:
- C8H10N2O2
- IUPAC Name:
- pyridin-3-yl N,N-dimethylcarbamate
Constituent 1
- Specific details on test material used for the study:
- - CAS No.: 51581-32-9
- Batch No.: TMFP 304
- Purity: At least 99 %
- Solubility in water: Soluble
- Storage: In the refrigerator, in the dark
- Date of expiry: December 1999
- Appearance: Colorless liquid
- pH of the test substance: 7.29 (10 % solution in deionised water, v/v, determined with a pH-meter)
Test animals / tissue source
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- - Supplier: J. Schitkovits, A-2620 Natschbach
- Justification for selection of the species: Recommended by the Directive / Guideline
- Number and sex: 3 females
- Body weight (at the start and at the termination of the test): Animal No. 11: 3.0 kg and 3.5 kg, Animal No. 12: 3.2 kg and 3.6 kg, Animal No. 13: 3.2 kg and 3.k - Housing: Individual caging in metal wire cages
- Diet (e.g. ad libitum): Yes
- Water (e.g. ad libitum): Yes
- Acclimation period: 10 days (animal no. 11) and 16 days (animal no. 12 and no. 13)
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21
- Humidity (%): 53
- Air changes (per hr): 12
- Photoperiod (hrs dark / hrs light): 12 / 12
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- yes, concurrent no treatment
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.1 mL - Duration of treatment / exposure:
- The test substance was administered to the conjunctival sac of the right eye by gently pulling the lower lid away from the eyeball for instillation. The eye was held closed for about one second to prevent loss of test substance.
- Observation period (in vivo):
- Both eyes of the animals were examined within 24 hours before instillation and approximately 1, 24, 48 and 72 hours p.a.
Additional examinations were performed 6, 8, 10, 13, 15, 17 and 21 days after the instillation, ending individually for each animal. - Number of animals or in vitro replicates:
- 3
- Details on study design:
- SCORING SYSTEM:
The scoring terms of 24, 48 and 72 h after the instillation were used
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 0.7
- Max. score:
- 4
- Reversibility:
- fully reversible within: 13d
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 1
- Max. score:
- 4
- Reversibility:
- not fully reversible within: 21d
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 1
- Max. score:
- 4
- Reversibility:
- fully reversible within: 6d
- Irritation parameter:
- iris score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 2
- Irritation parameter:
- iris score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 1
- Max. score:
- 2
- Reversibility:
- fully reversible within: 10d
- Irritation parameter:
- iris score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 1
- Max. score:
- 2
- Reversibility:
- fully reversible within: 6d
- Irritation parameter:
- conjunctivae score
- Remarks:
- redness
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 1
- Max. score:
- 3
- Reversibility:
- fully reversible within: 13d
- Irritation parameter:
- conjunctivae score
- Remarks:
- redness
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 2.7
- Max. score:
- 3
- Reversibility:
- not fully reversible within: 21d
- Irritation parameter:
- conjunctivae score
- Remarks:
- redness
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 2
- Max. score:
- 3
- Reversibility:
- fully reversible within: 8d
- Irritation parameter:
- chemosis score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 2
- Max. score:
- 4
- Reversibility:
- fully reversible within: 8d
- Irritation parameter:
- chemosis score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 3.7
- Max. score:
- 4
- Reversibility:
- fully reversible within: 17d
- Irritation parameter:
- chemosis score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 1
- Max. score:
- 4
- Reversibility:
- fully reversible within: 6d
- Irritant / corrosive response data:
- Ocular lesions were reversible in 2 / 3 animals within 17 days p.a. and were not reversible in 1 / 3 animals within 21 days
- Other effects:
- Other observations:
- Cornea: Diffuse areas of opacity (score "1") raised to easily discernible translucent areas with details of iris slightly obscured (score "2"). Most of the time the whole cornea was affected. White coverings on the sclera were noted in one animal 72 h p.a. and corneal lesions were still present 21 d p.a. In 2 / 3 animals, lesions were fully reversible. The corneae became normal again 6 d - 13 d p.a.
- Iris. In 2 / 3 animals mild changes (score "1") were noted from 24 h p.s. on and lasted until a maximum of 8 d p.a.
- Conjunctivae, redness: Injected blood vessels to diffuse beefy red colour were noted in all animals. The lesions were observed from 1 h p.a. to a maximum of 10 d p.a. in 2 / 3 animals and lasted until 21 d p.a. in the third animal. In 1/ 3 animals, a turbid ocular discharge from the instilled eye was seen 48 h p.a.
- Conjunctivae, chemosis: In all animals swelling in different degrees of severity were noted from 1 h onwards. The lesions then gradually returned to normal and 6, 8 and 17 d p.a. no swellings were detectable.
Applicant's summary and conclusion
- Interpretation of results:
- Category 1 (irreversible effects on the eye) based on GHS criteria
- Conclusions:
- In this study, Pyridobase caused irreversible serious ocular lesions in 1 / 3 animals and was irritating to the eyes in 2 / 3 animals.
- Executive summary:
In an in vivo eye irritation study conducted according to the OECD guideline 405, 0.1 mL of the test item was instilled to the conjunctival sac of one eye of each of 3 New Zealand White rabbits and the other eye was used as a control. The eyes were observed for body weights, general signs of toxicity and eye examinations. Irreversible ocular lesions in 1 / 3 animals and irritation in 2 / 3 animals was observed. Based on these results, the test substance is considered as an irritant under 'Category 1' according to the UN GHS Criteria.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.