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EC number: 617-357-9 | CAS number: 825633-75-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to soil macroorganisms except arthropods
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- May 2, 2013 - Dec. 31, 2013
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- no OECD GLP
- Justification for type of information:
- This study was performed according OECD TG 207. The quality system applied was not OECD GLP.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
- GLP compliance:
- no
- Remarks:
- no OECD GLP
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: L012138874
- Expiration date of the lot/batch: 2014-10-31
- Purity: > 99.92 % - Analytical monitoring:
- no
- Vehicle:
- yes
- Remarks:
- Acetone
- Details on preparation and application of test substrate:
- Artificial soil test substrate was prepared as follows:
a) 10 per cent sphagnum peat (pH 6.0, no visible plant remains, finely ground, dried).
b) 20 per cent kaolin clay (kaolinite content preferably above 30 per cent).
c) 70 per cent industrial sand (fine sand should be dominant with 60 per cent of the
particles between 50 and 200 microns).
d) pH is adjusted to 6.0 by addition of calcium carbonate.
The dry constituents were blended in the correct proportions and mixed thoroughly with a
large-scale laboratory mixer.
Test subsance amount was calculated based on dry weight - Test organisms (species):
- Eisenia fetida
- Details on test organisms:
- TEST ORGANISM
- Common name: Eisenia fetida
- Source: Earthworm nursery of Nanjing city in China
- Breeding generation: E20130415
- Age at test initiation (mean and range, SD): 2 month
- Weight at test initiation (mean and range, SD): 397 mg
ACCLIMATION
- Acclimation period: 24 h
- Acclimation conditions (same as test or not): no feeding
- Health during acclimation (any mortality observed): no mortality, no other findings - Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- yes
- Total exposure duration:
- 14 d
- Test temperature:
- 20-22 °C
- pH:
- 5.78 - 5.84
- Moisture:
- 35% dry soil weight
- Details on test conditions:
- TEST SYSTEM
- Test container (material, size): 1 L glass vessel, closed
- Amount of soil or substrate: 750 g
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4
- No. of replicates per vehicle control: 4
OTHER TEST CONDITIONS
- Photoperiod: 24 h/day
- Light intensity: 400 - 800 Lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
day 0 and 14
VEHICLE CONTROL PERFORMED: yes
TEST CONCENTRATIONS
1000 mg/kg dw - Nominal and measured concentrations:
- 1000 mg/kg dw
- Reference substance (positive control):
- no
- Key result
- Duration:
- 14 d
- Dose descriptor:
- LD50
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- The mortalities of the blank control group, the solvent control group and the treatment group on day 14 were 0%.
- Results with reference substance (positive control):
- LD50 at about 25 mg/kg soil dw
- Reported statistics and error estimates:
- Standard statistical methods have been applied for data processing.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under conditions of the test, the 14 -day LD50 for the test substance to earthworms (Eisenia fetida) based on nominal test concentrations were higher than 1000 mg/kg dry soil weight.
- Executive summary:
Study Design
In this study, the acute toxicity of the test substance to Eisenia fetida was determined in a 14 days artificial soil test. The study design followed the "OECD, Guidelines for the Testing of Chemicals, 1984, 207 Earthworm, Acute Toxicity Test".
Based on the results of range-finding test, one treatment group, one blank control group, and one solvent control group which had four replicates of 10 worms per concentration each were included in limit test. The nominal concentration of the treatment group was 1000 mg/kg dry artificial soil weight. The number of mortalities were determined after 14 days exposure.
Results
There was no mortality of the earthworms in the control group, therefore the test was valid.
After test material exposure, there was no mortality obvserved.
Conclusion
Under conditions of the test, the 14 -day LD50 for the test substance to earthworms (Eisenia fetida) based on nominal test concentrations were higher than 1000 mg/kg dry soil weight.
Reference
Description of key information
The informtion for this endpoint study record was obtained from an experimental study. The GLP (China) criteria were met and the methods applied are fully compliant with OECD TG 207. Under conditions of the test, the 14 -day LD50 for the test substance to earthworms (Eisenia fetida) based on nominal test concentrations were higher than 1000 mg/kg dry soil weight.
Key value for chemical safety assessment
- Short-term EC50 or LC50 for soil macroorganisms:
- 1 000 mg/kg soil dw
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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