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Short-term toxicity to aquatic invertebrates

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Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
25th june 2002 - 27th june 2002
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods
Qualifier:
equivalent or similar to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Version / remarks:
not specified explicitly in text
GLP compliance:
not specified
Analytical monitoring:
no
Details on sampling:
- Concentrations: 0 and 100 mg/L
Vehicle:
yes
Details on test solutions:
prepared in ISO , prepared in milli-RO water
Test organisms (species):
Daphnia magna
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
not specified
Test temperature:
19.9°C
pH:
7.7 - 8.0
Dissolved oxygen:
7.8 - 9.1 mg/L
Salinity:
not specified
Conductivity:
not specified
Nominal and measured concentrations:
nominal 0 and 100 mg/L
Details on test conditions:
TEST SYSTEM
- Type (delete if not applicable): closed
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
Reference substance (positive control):
not specified
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
one daphnid observed trapped at the surface of the test solutions. these daphnids were re-immersed in the respective solutions before scoring of mobility
Results with reference substance (positive control):
not specified
Reported statistics and error estimates:
not specified
Validity criteria fulfilled:
not specified
Conclusions:
Under the conditions of the the study, 8135C did not induce acute immobilisation of daphnia magna in a WAF loaded at 100 mg/L after 48 hours of exposure. The 48h EC50 was > a WAF loaded at 100mg/L.
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
24th june 2003 - 26th june 2003
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
not specified
Specific details on test material used for the study:
Pigment red 81:5/4 (silicomolybdic acid salt of C.I. 45160:4)
Red powder
Batch: pigment red 81:5/4
Analytical monitoring:
no
Details on sampling:
- Concentrations: 0.01, 0.1, 1, 10 mg/L
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stock solutions were prepared in acetone at a concetration of a factor of 10,000 higher than the final test solutions
- Eluate: 0.1mL of the stock solution was added per litre of test medium
- Differential loading: water accommodated fractions
- Controls: contain same amount of solvent as test item
Test organisms (species):
Daphnia magna
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
not specified
Test temperature:
20.2 - 21.0°C
pH:
7.6 - 7.8
Dissolved oxygen:
remained above 7mg/L
Salinity:
not specified
Conductivity:
not specified
Nominal and measured concentrations:
nominal 0.01, 0.1, 1, 10 mg/L
Reference substance (positive control):
no
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
< 0.01 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Results with reference substance (positive control):
none specified
Validity criteria fulfilled:
not specified
Conclusions:
The 48h EC50 was below 0.01mg/L
According to EEc directive 98/98, pigment red 81:5/4 should be classified as highly toxic to aquatic organisms
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
24th june 2003 - 26th june 2003
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
not specified
Specific details on test material used for the study:
pigment red 81:5/3
silicomolybdic acid salt of C.I 45160:4
Analytical monitoring:
no
Details on sampling:
concentrations: 0.01, 0.1, 1, 10 mg/L
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stock solutions prepared in acetone
- Eluate: water acommodated fractions
- Controls: yes, concurrent without test item
Test organisms (species):
Daphnia magna
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
not specified
Test temperature:
19.3 - 20.6°C
pH:
7.7 - 7.9
Dissolved oxygen:
remained above 7 mg/L
Salinity:
not specified
Conductivity:
not specified
Nominal and measured concentrations:
nominal: 0.01, 0.1, 1, 10 mg/L
Reference substance (positive control):
no
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
< 0.01 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Results with reference substance (positive control):
none specified
Reported statistics and error estimates:
not specified
Validity criteria fulfilled:
not specified
Conclusions:
In conclusion the 48h EC50 was below 0.01 mg/L.
According to EEC directive 98/98, pigment red 81:5/3 should be classified as highly toxic to aquatic organisms

Description of key information

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
1 mg/L

Additional information

EL50 value taken from the long term toxicity study to aquatic invertebrates (BQ63Y)