Registration Dossier
Registration Dossier
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EC number: 224-152-4 | CAS number: 4216-02-8
Endpoint summary
Administrative data
Description of key information
All aquatic toxicity studies were performed with the Source Substance C.I. Pigment Orange 43.
The acute toxicity of the test item to fish (zebrafish) was determined according to OECD-Guideline for Testing of Chemicals No. 203 (1992). A static limit test was conducted with a saturated solution prepared from a nominal concentration of 1.00 mg/L of the test item.
The saturated solution of the test item didn´t show any signs of toxicity nor lethality at all observation time points up to 96 hours. Therefore, the NOEC and LC0were laid down as 1.00 mg/L (based on the nominal concentration) and LC100was laid down as > 1.00 mg/L (based on the nominal concentration).
The acute toxicity of the test item to aquatic invertebrates (Daphnia magna) was determined according to OECD-Guideline for Testing of Chemicals No. 202 (2004). A static limit test was conducted with a saturated solution prepared from a nominal concentration of 1.00 mg/L of the test item.
The saturated solution of the test item didn´t show any signs of toxicity nor lethality at all observation time points up to 48 hours. Therefore, the NOEL was laid down as 1.00 mg/L (based on the nominal concentration) and EL100was laid down as > 1.00 mg/L (based on the nominal concentration).
The toxicity of the test item to the unicellular freshwater green alga (Desmodesmus subspicatus) was determined according to OECD 201 (2011). The study was conducted under static conditions with a saturated solution prepared from a nominal concentration of 1.00 mg/L of the test item. In this study the test item did not cause any inhibiting effects to Desmodesmus subspicatus after 72 h.
The No Observed Effect Concentration (NOEC) was assessed as 1.00 mg/L and the EC50 was laid down as > 1.00 mg/L (based on the nominal concentration)
A respiration inhibition test with activated sludge according to OECD 209 (2010) was performed under static conditions with the nominal limit concentration of 1000 mg/L. The test item caused no inhibiting effects at a concentration of 1000 mg/L to activated sludge of a municipal sewage treatment plant. The NOEC is 1000 mg/L.
Additional information
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