Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2015
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study performed according to OECD Guideline and following the priciples of Good Laboratory Practice

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2015
Report Date:
2015

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder
Details on test material:
batch number: 492-14-01.
the purity is 99.08 area % (190 nm) and 98.17 area % (200 nm)

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
The test organism, Daphnia magna Straus originated from the standard laboratory culture cultivated at the Institute of Industrial Organic Chemistry, Branch
Pszczyna, Department of Ecotoxicology[SOP/W/67]. Only organisms up to 24 hours old (not first brood progeny) and in good physiological
condition were used in the tests.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Test temperature:
19.1 – 20.4ºC
pH:
7.28 – 7.30
Dissolved oxygen:
9.00 – 9.01 mg/L (initial)
8.75 – 8.76 mg/L (final)
Nominal and measured concentrations:
120 mg/L, plus the control
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 120 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
The LOEC/48 h is higher than the test item concentration of 120 mg/L.
The NOEC/48 h is higher than or equal to the test item concentration of 120 mg/L.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes