Registration Dossier

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
April 2015
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study performed according to OECD Guideline and following the priciples of Good Laboratory Practice

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2015
Report Date:
2015

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder
Details on test material:
NM01; batch no.: 492-14-01
Purity: 99.08 area % (190 nm) and 98.17 area % (200 nm)

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
In the definitive test following test item concentrations were used: 31.3, 62.5, 125, 250 and 500 mg/L
plus the control (with a spacing factor of 2.0).

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h

Test conditions

Test temperature:
22.0 – 22.4°C
pH:
The pH values were in the ranges of 7.14 – 7.28 at exposure initiation and 8.11 – 8.58 at exposure termination
Nominal and measured concentrations:
In the definitive test following test item concentrations were used: 31.3, 62.5, 125, 250 and 500 mg/L
plus the control (with a spacing factor of 2.0).
Reference substance (positive control):
yes
Remarks:
3,5 dichlorophenol

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate

Any other information on results incl. tables

The EyC50/72 h is higher than the test item concentration of 500 mg/L.

The EyC10/72 h is 264 mg/L (95% confidence limits were not determined).

Applicant's summary and conclusion

Validity criteria fulfilled:
yes