Estrone

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro cytogenicity / chromosome aberration study in mammalian cells

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

Inducibility of chromosome aberrations by steroid hormones in cultured chinese hamster ovary cells

Author:

Kochhar T.S.

Year:

1985

Bibliographic source:

Toxicology Letters 29, 201-206 (1985)

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

CHO cells used in the current investigation were routinely grown in 75cm² Corning plastic flasks in 10 ml of McCoy’s 5a medium supplemented with 10% fetal calf serum and antibiotics consisting of penicillin-G, (100 U/ml) and streptomycin, (100 µg/ml). The cultures were incubated in an atmosphere of 5% CO2 in air. To test the effect of steroid hormones, cultures were set up 24 h prior to the treatment by seeding approx. 1.6 x 10 to 6 cells per flask. Treatments were given by exposing the cultures to test compounds dissolved in DMSO and suspended in the culture medium for 12 h. The control cultures contained 0.5% of DMSO and were incubated under identical conditions. The procedure for the preparation of chromosomes to score for the abnormalities
is described in Experientia, 38 (1982) 845-846.

GLP compliance:

not specified

Type of assay:

mammalian cell gene mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Estrone

EC Number:

200-164-5

EC Name:

Estrone

Cas Number:

53-16-7

Molecular formula:

C18H22O2

IUPAC Name:

estrone

Specific details on test material used for the study:

The test material was procured from Sigma, St. Louis, MO.

Method

Species / strain

Species / strain / cell type:

Chinese hamster Ovary (CHO)

Details on mammalian cell type (if applicable):

The CHO cells were obtained from Environmental Health Research and Testing, Lexington, KY. The cells were not used at a passage level of more than 15 after cloning, and were thawed routinely from liquid nitrogen storage and maintained by transferring twice a week.

Metabolic activation:

not specified

Test concentrations with justification for top dose:

10 to minus five M; 5 x 10 to minus five M; 10 to minus 4 M

Vehicle / solvent:

DMSO

Controls

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

no

Results and discussion

Test results

Species / strain:

Chinese hamster Ovary (CHO)

Metabolic activation:

not applicable

Genotoxicity:

positive

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

valid

Untreated negative controls validity:

not applicable

Positive controls validity:

not examined

Applicant's summary and conclusion

Conclusions:

positive

Executive summary:

The effect of steroid hormones on the chromosomes of cultured Chinese hamster ovary (CHO) cells was studied. It was noticed that estradiol-17fl, estrone, estriol and ethynyl estradiol were effective in producing various types of chromosome aberrations. The percentage of these abnormalities increased with increasing concentrations of steroids used.