Registration Dossier

Administrative data

Endpoint:
genetic toxicity in vitro
Remarks:
Type of genotoxicity: other: NUCLEIC ACID SYNTHESIS
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Data is from peer reviewed journal

Data source

Reference
Reference Type:
publication
Title:
A COMPARATIVE STUDY OF THE EFFECTS OF MERCURY COMPOUNDS ON CELL VIABILITY AND NUCLEIC ACID SYNTHESIS IN HeLa CELLS
Author:
EDDIE S-E. CHAO, JOHN F. GIERTHY and GERALD D. FRENKEL
Year:
1984
Bibliographic source:
Biochemical Pharmacology, Vol. 33, No. 12, pp. 1941-1945, 1984

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: as mentioned below
Principles of method if other than guideline:
Genetic toxicity test was performed on HeLa cells
GLP compliance:
not specified
Type of assay:
other: No data available

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material: Mercury sulphate
- Molecular formula: H2O4S.Hg
- Molecular weight : 298.67 g/mol
- Smiles notation: [O-]S(=O)(=O)[O-].[Hg+2]
- InChl : InChI=1S/Hg.H2O4S/c;1-5(2,3)4/h;(H2,1,2,3,4)/q+2;/p-2
- Substance type: Solid
- Physical state: Inorganic
- Impurities (identity and concentrations): No data

Method

Target gene:
HeLa cells
Species / strain
Species / strain:
other: HeLa cells
Details on mammalian cell lines (if applicable):
Details on mammalian cell line
- Type and identity of media: Dulbecco's Modified Eagle's Medium
- Properly maintained: yes
- Periodically checked for Mycoplasma contamination: No data available
- Periodically checked for karyotype stability: No data available
- Periodically "cleansed" against high spontaneous background: No data available
Additional strain characteristics:
not specified
Metabolic activation:
not specified
Metabolic activation system:
no data
Test concentrations with justification for top dose:
No data available
Vehicle:
No data available
Controls
Negative controls:
not specified
Solvent controls:
not specified
True negative controls:
not specified
Positive controls:
not specified
Positive control substance:
not specified
Remarks:
no data
Details on test system and conditions:
No data available
Evaluation criteria:
inhibition concentration
Statistics:
No data available

Results and discussion

Test results
Species / strain:
other: Hela cells
Metabolic activation:
not specified
Genotoxicity:
positive
Cytotoxicity:
yes
Vehicle controls valid:
not specified
Negative controls valid:
not specified
Positive controls valid:
not specified
Remarks on result:
other:
Remarks:
Migrated from field 'Test system'.
Additional information on results:
no data

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
positive

The genetic toxicity for Mercuric sulfate (7783-35-9) was found to be positive in Hela cells.
Executive summary:

The HeLa cells were grown on monolayer cultures in Dulbecco's Modified Eagle's Medium. Three parameters were tested for study the effect of mercury sulphate as DNA polymerase α was purified from HeLa cells, Nucleic acid synthesis and intact cells. Mercury sulphate and radiolabelled precursor were added and radioactivity was measured after incubation for 30 min at 37°C. Experiment was conducted in duplicates.

Cytotoxicity was also measured in HeLa cells HeLa cells were seeded into

50 mm dishes at a concentration of 360 cells per dish, with 3.6 ml of Dulbecco's Modified Eagle's Medium with 10% heat-inactivated fetal calf serum. After incubation of the cultures at 37°C for 24 hr, 0.4 ml of a series of 2-fold dilutions of a freshly made 2 mM solution of the mercury compound was added to the cultures. After 9 days of further incubation the cultures were washed with 4ml of phosphate buffered saline (pH 7.6), fixed with methanol, and stained with Giemsa, and the number of colonies per dish was determined. Six replicates were done for each concentration.

RNA synthesis in intact cells was inhibited by mercury sulphate.

As the IC 50 value forDNA polymerase α, Intact cells, Isolated Nuclei was found and mercury sulphate as also cytotoxic effect so on the basis of the result it was found thatgenetic toxicity for Mercuric sulfate (7783-35-9) was found to be positive in HeLa cells.