3'-aminoacetophenone

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data from peer review publication

Data source

Reference

Reference Type:

publication

Title:

The Induction of Bacterial Mutation and Hepatocyte Unscheduled DNA Synthesis by Mono-substituted Anilines

Author:

Christina Z. Thompson, Leo E. Hill, Janet K. Epp, and Gregory S. Probst

Year:

1983

Bibliographic source:

Environmental Mutagenesis 5:803-811 (1983)

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

Gradient Plate Test for Bacterial Mutation

GLP compliance:

no

Type of assay:

bacterial forward mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

3'-aminoacetophenone

EC Number:

202-722-3

EC Name:

3'-aminoacetophenone

Cas Number:

99-03-6

Molecular formula:

C8H9NO

IUPAC Name:

1-(3-aminophenyl)ethan-1-one

Details on test material:

Name of test material (as cited in study report): m-aminoacetophenone
Substance type: Organic
Physical state: Liquid
Purchased from: Aldrich Chemical Company,
Inc. (Milwaukee, WI) or from Eastman Chemical Company (Rochester, NY).

Method

Target gene:

Plate Incorporation
S typhimurium (G46, TA 1535, TA 100, C3076,
TA1537, D3052, TA1538, and TA98) and two tryptophan auxotrophs of E coli (WP2
and WF2uvrA-).

Species / strain

Species / strain / cell type:

other: S typhimurium (G46, TA 1535, TA 100, C3076, TA1537, D3052, TA1538, and TA98) and two tryptophan auxotrophs of E coli (WP2 and WF2uvrA-).

Details on mammalian cell type (if applicable):

Not applicable

Additional strain / cell type characteristics:

not specified

Metabolic activation:

with and without

Test concentrations with justification for top dose:

0-1000 µg/plate

Vehicle / solvent:

Vehicle used: DMSO

Controls

Untreated negative controls:

not specified

Negative solvent / vehicle controls:

yes

True negative controls:

not specified

Positive controls:

yes

Positive control substance:

other: Streptozotocin (STZ) (Sigma Chemical Company, St. Louis, MO) and 2-acetylaminofluorene (2-AAF) (Aldrich Chemical Company, Milwaukee, WI) served as positive controls for the bacterial mutation assay.

Details on test system and experimental conditions:

METHOD OF APPLICATION :
in agar (plate incorporation)
DURATION
No data
SELECTION AGENT (mutation assays):No data
SPINDLE INHIBITOR (cytogenetic assays):No data
STAIN (for cytogenetic assays):No data
NUMBER OF REPLICATIONS: No data
NUMBER OF CELLS EVALUATED:
No data

Evaluation criteria:

The results indicate the number of strains
showing chemically induced revertant colonies as well as the concentration range
over which mutation was observed in the most sensitive strain.

Results and discussion

Test results

Species / strain:

other: S typhimurium (G46, TA 1535, TA 100, C3076, TA1537, D3052, TA1538, and TA98) and two tryptophan auxotrophs of E coli (WP2 and WF2uvrA-).

Metabolic activation:

with and without

Genotoxicity:

positive

Cytotoxicity / choice of top concentrations:

no cytotoxicity

Vehicle controls validity:

valid

Untreated negative controls validity:

not valid

Positive controls validity:

valid

Additional information on results:

No data

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
positive Positive with and without metabolic activation in S typhimurium TA100, TA98,TA1538

Mono- substituted aniline derivatives were tested for the induction of bacterial mutation using eight S typhimurium and two E coli strains. The gradient plate test for bacterial mutation was conducted with and without metabolic activation.
m-amino acetophenone exhibited mutagenic activity with and without metabolic activation in S typhimurium TA100, TA98,TA1538 strains
The Mutagenic concentration rang (MCR) in µg/ml of agar for the positive strains is 10-1000.

Executive summary:

Mono- substituted aniline derivatives were tested for the induction of bacterial mutation using eight S typhimurium and two E coli strains. The gradient plate test for bacterial mutation was conducted with and without metabolic activation.All compounds were tested as supplied without further purification or attempt to identify impurities. Streptozotocin (STZ) (Sigma Chemical Company,St.Louis, MO) and 2-acetylaminofluorene (2-AAF) (Aldrich Chemical Company, Milwaukee, WI) served as positive controls for the bacterial mutation assay.

This test employed ten bacterial strains: eight histidine auxotrophs of S typhimurium (G46, TA 1535, TA100,C3076, TA1537, D3052, TA1538, and TA98) and two tryptophan auxotrophs of E coli (WP2 and WF2uvrA-). Each compound was incorporated into the top wedge of agar on each of four square plates to give a 10-fold concentration gradient per plate and thus provide a 10,000-fold concentration range for the compound. All tests were conducted with and without Aroclor 1254-induced rat liver S-9 and a maximum compound concentration of 1000 µg/ml of agar.

The results indicate the number of strains showing chemically induced revertant colonies as well as the concentration range over which mutation was observed in the most sensitive strain.

m-amino acetophenone exhibited mutagenic activity with and without metabolic activation in S typhimurium TA100, TA98,TA1538 strains

The Mutagenic concentration rang (MCR) in µg/ml of agar for the positive strains is 10-1000