2-Propenoic acid, 2-methyl-, 2-[2-(4-benzoylphenoxy)ethoxy]ethyl ester

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2015

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline Study with detailed documentation

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Report date:

2015

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

MAEEBP

IUPAC Name:

MAEEBP

Details on test material:

- Name of test material (as cited in study report): MAEEBP- Physical state: colourless liquid- Composition of test material, percentage of components: 4-methacryloyl diethoxy benzophenone 85 area%, impurities 4-hydroxybenzophenone <5%, 4-methacryloyl benzophenone <5%, polymethacrylate <5%- Lot/batch No.: 150301- Expiration date of the lot/batch: Sep. 2015- Storage condition of test material: The test item was stored in the test facility in a closed vessel in the refrigerator (3.9-9.2°C) (exception: 22.- 28. Apr. 2015 storage at room temperature).

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge: Activated sludge from a biologic sewage treatment plant of the ESN (Stadtentsorgung Neustadt), Germany- Pre-treatment: The sludge was filtrated, washed with tap water twice, then washed with and re-suspended in test medium. It was then aerated until use.- Concentration of sludge: 4540 mg suspended solids/L (dry matter)

Duration of test (contact time):

29 d

Initial test substance concentration

Initial conc.:

20 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

TEST SYSTEM- Culturing apparatus: 2000 mL-SCHOTT-flasks were used as test vessels, 100 mL scrubber flasks as absorbent vessels. Flask volume 1500 mL- Number of culture flasks/concentration: 2- Method used to create aerobic conditions: The test vessels were filled with medium and inoculum. Then, all flasks were aerated for 72 hours with purified, CO2-free, moistened air to purge the system of CO2.- Measuring equipment: carbon analyser TOC multi N/C 2100S, Analytik JenaSAMPLING- Sampling frequency: on day 0, 2, 4, 7, 9, 11, 14, 18, 23 and 29CONTROL AND BLANK SYSTEM- Inoculum blank: 2 flasks- Reference: 2 flasks- Abiotic sterile control: 1 flask (HgCl2)- Toxicity control: 1 flask - Other: Apparatus blanks, 2 flasks, containing mineral medium only

Reference substance

Reference substance:

aniline

Results and discussion

% Degradation

Parameter:

% degradation (CO2 evolution)

Value:

43

Sampling time:

28 d

Any other information on results incl. tables

Degradation values after 29 d:

Positive control mean: 86.8%

Test mean: 42.7%

Abiotic control: -1.4%

Toxicity control: 53.7%

10-day-window: day 12 – 22

Degradation at the end of 10-day-window 25 %

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

inherently biodegradable

Conclusions:

The test item MAEEBP was tested using a concentration of nominally 20 mg organic carbon/L (corresponding to 28.5 mg MAEEBP/L) in test medium following OECD 301B and EU-Method C.4-C. The test item degraded to 43% within 28 days. Therefore, regardless of the 10-day-window, MAEEBP is not readily biodegradable following OECD 301B/EU C.4-C.