Anilinoacetonitrile

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DIN 38412, Part 15

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Anilinoacetonitrile

EC Number:

221-129-0

EC Name:

Anilinoacetonitrile

Cas Number:

3009-97-0

Molecular formula:

C8H8N2

IUPAC Name:

2-(phenylamino)acetonitrile

Details on test material:

Phenylglycinnitril
Test Substance No.: 88/718
Purity: about 100%

Solubility in water: about 5 g/L

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

The product was added to the test water without any pretreatment. No undissolved test substance was visible.

Test organisms

Test organisms (species):

Leuciscus idus

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Test conditions

Nominal and measured concentrations:

Based on the results of a range finding study (LC50 after 96 h: Between 10 and 100 mg/L) the concentrations were fixed as follows: 10.0, 21.5, 46.4, 100 and 215 mg/L.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

46.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

46.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

68.12 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

Results presented as nominal values.
During the test no mortality occured in the control group as well as in the test substance concentration groups up to 46.4 mg/L. One and 9 dead fish were observed at test concentrations of 100 mg/L and 215 mg/L after 24 h of exposure, respectively. After 48 hours of exposure 7 fish died at a test concentration of 100 mg/L and no living fish were found at 215 mg/L. After 72 h all test animals were dead in the 100 mg/L test concentration group.
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No signs of intoxication were observed in the control group as well as in the test concentration groups up to 46.4 mg/L.
Exposed to 100 mg/L fish showed apathy and tumbling starting after 4 hours of exposure. In the test group exposed to 215 mg/L tumbling started after 1 hour of exposure followed by convulsions and narcotic-like states after 4 and 24 hours.
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LC50 presented as geometrical mean value