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Diss Factsheets
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EC number: 233-139-2 | CAS number: 10043-35-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to aquatic plants other than algae
Administrative data
- Endpoint:
- toxicity to aquatic plants other than algae
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Peer-reviewed technical publication that meets basic scientific principles.
Data source
Reference
- Reference Type:
- publication
- Title:
- Toxicity of boron to the duckweed, Spirodella polyrrhiza
- Author:
- Davis, SM, KD Drake and KJ Maier
- Year:
- 2 002
- Bibliographic source:
- Chemosphere 48, 615-620
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EPA OPPTS 850.4400 (Aquatic Plant Toxicity Test using Lemna spp. Tiers I & II))
- Deviations:
- yes
- Remarks:
- no further details available
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Boric acid
- EC Number:
- 233-139-2
- EC Name:
- Boric acid
- Cas Number:
- 10043-35-3
- Molecular formula:
- H3BO3
- IUPAC Name:
- boric acid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- On day 1, 4, 7 and 10, three 24h samples for each concentration were collected.
All samples were stored at 4°C until analysis.
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- other: Spirodella polyrrhiza
- Details on test organisms:
- TEST ORGANISM
- Common name: Duckweed
- Source : existing laboratory culture
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 10 d
Test conditions
- Test temperature:
- 25 +- 1 °C
- Nominal and measured concentrations:
- Nominal concentrations : 0.5, 3, 6, 17, 25 and 37 mg B/L
To verify B exposure, water samples were analyzed using the colorimetric curcumin method as described by Allen (1974). Mean concentrations of time 0 and 24 hr samples were: 0.9 (control), 3.6, 6.5, 19.2, 24.0, and 40.0 mg B/L. B concentrations for days 4, 6 and 10 were not reported. Authors expressed endpoints based on time 0 concentrations, but mean values are used below. - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 50 ml plastic cups filled with 45 ml of boron-containing nutrient media
- Renewal rate of test solution (frequency/flow rate): daily
- No. of fronds per colony: 14-16
- No. of vessels per concentration (replicates): 6
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: pH stabilized nutrient media (Clatworthy and Harper, 1962)
OTHER TEST CONDITIONS
- Photoperiod, light intensity and quality: 24h light from normal chamber illumination + two 24-in. Lampi Flora Plant lights each fitted with 20 W wide-spectrum fluorescent lights were placed +- 12 in. above the test chambers.
EFFECT PARAMETERS MEASURED :
- Determination of frond number: On day 0 and 10,frond number was determined, and observations of fronds were made. Chlorosis, necrosis and death were observed and classified as abnormal fronds.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 10 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 3.6 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- element
- Basis for effect:
- frond number
- Duration:
- 10 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 6.5 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- element
- Basis for effect:
- growth rate
- Duration:
- 10 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 19.5 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- element
- Basis for effect:
- growth rate
- Details on results:
- Total frond production reduced at next higher exposure (3.6 mg B/L) so no NOEC for total frond number can be derived. EC10 values could not be calculated
- Reported statistics and error estimates:
- Statistical analysis was performed using TOXSTAT 3.5 and ANOVA. The ANOVA and Tukey's HSD were performed to measure statistical differences between boron concentrations on days 0 and 10. These statistical tests were also used to analyze differences in initial concentrations and 24-h sample boron concentrations. Significance was determined using p =< 0.05 for all statistical tests.
Any other information on results incl. tables
Duration | Endpoint | Effect conc. | Nominal/Measured | Conc. based on | Basis for effect | Remarks (e.g. 95% CL) |
---|---|---|---|---|---|---|
10 d | LOEC | 3.6 mg/L | meas. (not specified) | element | frond number | unbounded LOEC |
10 d | EC50 | 14.3 mg/L | meas. (not specified) | element | frond number | |
10 d | NOEC | 6.5 mg/L | meas. (not specified) | element | growth rate | |
10 d | LOEC | 19.2 mg/L | meas. (not specified) | element | growth rate | |
10 d | EC50 | 11.7 mg/L | meas. (not specified) | element | growth rate |
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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