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EC number: 201-070-7
CAS number: 77-93-0
The mean biodegradation after 28 days of the test substance was 78%, the 10 day window criterion was passed from day 4 until day 14 (readily biodegradable). [OECD Guideline 301 F, 2010]
The biodegradability of the substance
triethyl citrate was investigated according to EU method C.4 -D and OECD
Guideline 301 F (2010). The study was conducted in compliance with the
Principles of Good Laboratory Practice (GLP). A measured volume of
inoculated mineral medium, containing the test item in a concentration
of 102 mg/L, which corresponds to an oxygen demand of about 160 mg/L
(ThODNH4), was stirred in a closed flask at a temperature of
22 °C for up to 28 days. Aerobic activated sludge, micro organisms from
a domestic waste water treatment plant was taken as inoculum. The
concentration of the inoculum was 26 mg/L. Analytical grade salts were
added to deionised water to prepare the stock solutions. Sodium benzoate
(purity of 100 %) was used as reference compound. Also a toxicity
control (test item and reference compound mixed) was run in parallel, to
ensure, that the chosen test concentration was not inhibitory to
microorganims. The degradation rate of the test item was calculated by
the oxygen consumption of the aerobic activated sludge microorganisms
after 28 days of incubation. As the test item contains no nitrogen, the
evaluation of biodegradation has to be based ThODNH4. The
criterion for ready biodegradability under the conditions of a
manometric respirometry test is the 10-day window, describing the period
between reaching at least 10 % degradation and 60 % degradation. This
period should not exceed 10 days. The mean biodegradation after 28 days
of the test substance was 78 % (ThODNH4), further the 10 day
window criterion was passed and therefore it is considered to be readily
biodegradable. The reference item sodium benzoate was sufficiently
degraded to 85 % after 14 days and to 88 % after 28 days of incubation,
thus confirming the suitability of the aerobic activated sludge inoculum
used. In the toxicity control containing both, the test item and the
reference item sodium benzoate, 54 % biodegradation was noted within 14
days and 67 % biodegradation after 28 days of incubation. Thus, the test
item can be assumed not to be inhibitory to the aerobic activated sludge
micro organisms and all validity criteria of the test method were met.
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