Methanesulphonic acid

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Comparable to OECD guideline with GLP, acceptable with restrictions.

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Reference 2

Reference Type:

publication

Title:

HPVIS, IUCLID Data Set for Methanesulphonic acid, CAS no. 75-75-2

Author:

US-EPA

Year:

2003

Bibliographic source:

HPV Chemicals Challenge Program OPPT NCIC 201-14249B

Materials and methods

Test guideline

Test guideline 1

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Version / remarks:

Limit dose: 1000 mg/kg

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: 49 CFR 173.132

GLP compliance:

yes

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Methanesulphonic acid

EC Number:

200-898-6

EC Name:

Methanesulphonic acid

Cas Number:

75-75-2

Molecular formula:

CH4O3S

IUPAC Name:

methanesulfonic acid

Details on test material:

- Name of test material (as cited in study report): methane sulfonic acid (MSA)
- Physical state: aqueous solution
- Composition of test material, percentage of components: 70.2 % in water
- Lot/batch No.: 20330PI
- Other: Source: Atofina Chemicals, Atofina, Paris La-Defense, France

Test animals

Species:

rabbit

Strain:

New Zealand White

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Millbrook Breeding Labs, MA
- Weight at study initiation: males 2.5-3.1 Kg, females 2.8-3.0 Kg
- Housing: 1 per cage
- Diet (e.g. ad libitum): Fresh PMI rabbit chow
- Water (e.g. ad libitum): drinking water
- Acclimation period: 1 week


ENVIRONMENTAL CONDITIONS
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Type of coverage:

occlusive

Vehicle:

water

Details on dermal exposure:

TEST SITE
- % coverage: 10
- Type of wrap if used: no data

REMOVAL OF TEST SUBSTANCE
- Washing (if done): Yes, distilled water
- Time after start of exposure: 24 hours

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 5 ml/kg bw
- Concentration (if solution): 200 mg/ml
- Constant volume or concentration used: yes

Duration of exposure:

24 hours

Doses:

1000 mg/kg bw

No. of animals per sex per dose:

10

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: clinical observations 1 and 2 hours postdose and once daily thereafter; dermal responses 24 hours postdose and on days 7 and 14; weighing pretest and at termination
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 1 000 mg/kg bw

Mortality:

No mortality occured, all animals survived the application of 1000 mg/kg bw Methane Sulfonic Acid.

Clinical signs:

other: Instances of few faces were the only abnormal physical signs noted during the observation period. Dermal responses were slight to well defined on day 1, absent to severe on day 7 and absent to slight on day 14.

Gross pathology:

Necropsy results were normal.

Any other information on results incl. tables

No necrosis reported under the study conditions chosen.