Methanesulphonic acid

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with GLP.

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1997

Report date:

1997

Reference 2

Reference Type:

publication

Title:

HPVIS, IUCLID Data Set for Methanesulphonic acid, CAS no. 75-75-2

Author:

US-EPA

Year:

2003

Bibliographic source:

HPV Chemicals Challenge Program OPPT NCIC 201-14249B

Materials and methods

Test guideline

Test guideline 1

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: EPA 40 CFR Part 792

GLP compliance:

yes (incl. QA statement)

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Methanesulphonic acid

EC Number:

200-898-6

EC Name:

Methanesulphonic acid

Cas Number:

75-75-2

Molecular formula:

CH4O3S

IUPAC Name:

methanesulfonic acid

Details on test material:

- Name of test material (as cited in study report): Methanesulphonic acid
- Physical state: clear colorless liquid
- Composition of test material, percentage of components: 70 % in water
- Lot/batch No.: D11HD1
- Other: Source: Elf Atochem

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Charles River Laboratories, Inc., Portage, Michigan
- Weight at study initiation: 205.3 g mean (males), 204.7 g mean (females)
- Diet (e.g. ad libitum): PMI certified Rodent Chow #5002
- Water (e.g. ad libitum): tap water
- Acclimation period: 5 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20-24
- Humidity (%): 38-61
- Air changes (per hr): 10-15
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

water

Details on oral exposure:

VEHICLE
- Amount of vehicle (if gavage): 0.38, 0.75, 1.13 ml/kg bw

MAXIMUM DOSE VOLUME APPLIED: 1.13 ml/kg bw

Doses:

500, 1000, 1500 mg/kg body weight

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: observations: no data; weighing: every 7 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, mortality, necropsy

Statistics:

The LD50 was determined separately for both sexes using the method of Litchfield and Wilcoxon.

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LD50

Effect level:

1 157.5 mg/kg bw

95% CL:

748 - 1 791.1

Remarks on result:

other: 70 % methane sulfonic acid

Effect levels 2

Sex:

male

Dose descriptor:

LD50

Effect level:

860.1 mg/kg bw

95% CL:

540.1 - 1 369.7

Remarks on result:

other: 70 % methane sulfonic acid

Effect levels 3

Sex:

female

Dose descriptor:

LD50

Effect level:

2 407.6 mg/kg bw

95% CL:

944.2 - 6 139.2

Remarks on result:

other: 70 % methane sulfonic acid

Mortality:

Males: 1/5, 3/5, and 4/5 deaths at 500, 1000, and 1500 mg/kg bw, respectively.
Females: 1/5, 0/5, and 3/5 deaths at 500, 1000, and 1500 mg/kg bw, respectively.

Clinical signs:

other: The most notable clinical abnormalities observed during the study included salivation, breathing abnormalities, wobbly gait, decreased activity, decreased defecation, rough haircoat, urine/fecal stain and dark material around the facial area.

Gross pathology:

The most notable gross internal findings were observed in the animals that died and included abnormal content and reddened/thickened mucosa and discolouration in the digestive tract, dark red foci on the liver and blackish-purple spleens. No significant gross findings were observed at necropsy on day 14.

Other findings:

- Potential target organs: Digestive tract

Any other information on results incl. tables

Table: Mortalities during 14 days of observation period

Dose group (mg/kg bw)	No. of animals	Study day														Mortality
		0	1	2	3	4	5	6	7	8	9	10	11	12	13-14
500	5 M	0	1	0	0	0	0	0	0	0	0	0	0	0	0	1/5
1000	5 M	3	0	0	0	0	0	0	0	0	0	0	0	0	0	3/5
1500	5 M	1	1	1	1	0	0	0	0	0	0	0	0	0	0	4/5
500	5 F	0	0	1	0	0	0	0	0	0	0	0	0	0	0	1/5
1000	5 F	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0/5
1500	5 F	1	1	1	0	0	0	0	0	0	0	0	0	0	0	3/5