Registration Dossier
Registration Dossier
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The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
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EC number: 203-311-1 | CAS number: 105-58-8
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- genetic toxicity in vitro
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: acceptable data from a publication with solid information on materials/methodology and results
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Salmonella Mutagenicity Tests: II. Results From the Testing of 270 Chemicals
- Author:
- Mortelmans, K. et al.
- Year:
- 1 986
- Bibliographic source:
- Environ Mutagen 8, Supplement 7, 1-119
- Reference Type:
- publication
- Title:
- Methods for detecting carcinogens and mutagens with the Salmonella/mammalian microsome mutagenicity test.
- Author:
- Ames et al.
- Year:
- 1 975
- Bibliographic source:
- Ames BN, McCann J, Yamasaki E (1975); Mutat Res 31:347-364
Materials and methods
- Principles of method if other than guideline:
- In this publication of Mortelmans et al. is referred to various publications including the essential publication of Ames et al. (1975) to describe and justifiy materials and methodology that were applied.
- GLP compliance:
- not specified
- Type of assay:
- other: Salmonella/mammalian microsome assay
Test material
- Reference substance name:
- Diethyl carbonate
- EC Number:
- 203-311-1
- EC Name:
- Diethyl carbonate
- Cas Number:
- 105-58-8
- Molecular formula:
- C5H10O3
- IUPAC Name:
- diethyl carbonate
- Details on test material:
- Name of the test substance as stated in the publication: diethyl carbonate
Purity: > 98 %
Purchased from: Aldrich
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- other: Salmonella TA1535, TA1537, TA98, TA100
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9-mix, rat and hamster liver
- Test concentrations with justification for top dose:
- 0, 100, 333, 1000, 3333, 6666 µg/plate
- Vehicle / solvent:
- Solvent dimethylsulfoxide (DMSO)
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: sodium azide (TA1535, TA100), 4-nitro-o-phenylenediamine (TA98), 9-aminoacridine (TA97, TA1537)
Results and discussion
Test results
- Species / strain:
- other: Salmonella TA1535, TA1537, TA98, TA100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
The test substance diethyl carbonate showed no mutagenicity on the different bacterial strains in the dose-level range from 0-6666 µg/plate.
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