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EC number: 203-311-1 | CAS number: 105-58-8
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Principles of method if other than guideline:
- A defined amount of activated sludge is combined with synthetic medium and the respiration rate is measured. This respiration rate is compared with the ones measured in the same experiment only using test medium with different levels of the test substance. The sensitiveness of the activated sludge is checked by one experiment using 3,5-dichlorophenol in the test medium. Non-biotic oxygen consumption was examined in addition by using the highest test substance level without addition of activated sludge.
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- activated sludge: mixed population of different microorganisms
source of activated sludge: laboratory activated sludge facility, connected to sewage water supply from "südlicher Wupperverband", Germany
pre-treatment of activated sludge: none - Total exposure duration:
- 30 min
- Remarks on exposure duration:
- under permanent aeration
- Test temperature:
- 20 +/- 2 °C
- pH:
- 6.3 (culture solution before beginning of respiration test)
- Nominal and measured concentrations:
- nominal (only): 1000, 1800, 3200, 5600, 10000 mg/l
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol (97 % purity)
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 000 mg/L
- Remarks on result:
- other: EC50 (diethyl carbonate pure)
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- 11 mg/L
- Remarks on result:
- other: EC50 (3,5-dichlorophenol): determined via Probit analysis, graphically determined using a half-logarithmic scale (Gauss Integral)
- Validity criteria fulfilled:
- yes
- Conclusions:
- The acute bacteria toxicity determined via a method basically in accordance with TG OECD 209 was EC50 > 10000 mg/l.
Reference
| Test substance and control (Table 1) |
| Sample | concentration (mg/l) | amount (mg) added to 250 ml | O2-start (mg O2/l) | O2-end (mg O2/l) | time (min) | temp. (°C) | pH |
| test substance | 1000 | 250 | 6.3 | 4.7 | 4 | 20.0 | 7.4 |
| test substance | 1800 | 450 | 6.0 | 4.1 | 5 | 20.5 | 7.3 |
| test substance | 3200 | 800 | 6.5 | 4.9 | 4 | 20.1 | 7.4 |
| test substance | 5600 | 1400 | 6.3 | 5.2 | 3 | 20.0 | 7.6 |
| test substance | 10000 | 2500 | 6.4 | 5.5 | 3 | 19.9 | 7.4 |
| control 1 | 5.6 | 4.3 | 3 | 19.7 | 7.7 | ||
| control 2 | 5.5 | 3.5 | 5 | 21.0 | 7.2 | ||
| abiotic O2 consumption | 10000 | 2500 | 8.3 | 8.3 | 10 | 19.5 | 7.3 |
| abiotic O2 consumption | |||||||
| abiotic O2 consumption | |||||||
| abiotic O2 consumption | |||||||
| abiotic O2 consumption |
| Reference substance 3,5-dichlorophenol (Table 1): |
| Sample | concentration (mg/l) | O2-start (mg O2/l) | O2-end (mg O2/l) | time (min) | temp. (°C) | pH |
| reference substance | 2.5 | 6.5 | 5.4 | 3 | 19.6 | 7.6 |
| reference substance | 5 | 6.3 | 5.2 | 3 | 19.8 | 7.4 |
| reference substance | 10 | 8.3 | 7.2 | 6 | 19.6 | 7.3 |
| reference substance | 20 | 8.6 | 8.1 | 5 | 19.9 | 7.3 |
| reference substance | 40 | 8.8 | 8.5 | 5 | 20.0 | 7.2 |
| Test substance and control (Table 2): |
| test substance concentration (mg/l) | respiration rate (mg/l*h) | abiotic O2 consumption (mg/l*h) | respiration rate - abiotic O2 consumption (mg O2/l*h) | inhibition (%) |
| 1000 | 24.0 | 0.0 | 24.0 | 4.0 |
| 1800 | 22.8 | 0.0 | 22.8 | 8.8 |
| 3200 | 24.0 | 0.0 | 24.0 | 4.0 |
| 5600 | 22.0 | 0.0 | 22.0 | 12.0 |
| 10000 | 18.0 | 0.0 | 18.0 | 28.0 |
| control mean value | 25.0 | |||
| control 1 | 26.0 | |||
| control 2 | 24.0 |
| Reference substance 3,5-dichlorophenol (Table 2): |
| test concentration (mg/l) | respiration rate (mg/l*h) | inhibition (%) |
| 2.5 | 22.0 | 12.0 |
| 5 | 22.0 | 12.0 |
| 10 | 11.0 | 56.0 |
| 20 | 6.0 | 76.0 |
| 40 | 3.6 | 85.6 |
Description of key information
Determined EC50 in toxicity-to-microorganisms study was ≥ 10000 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 10 000 mg/L
Additional information
The author of the toxicity-to-microorganisms study for the test substance diethyl carbonate evaluated a EC50 > 10000 mg/L.
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