2,2-bis(chloromethyl)trimethylene bis(bis(2-chloroethyl)phosphate)

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study without detailed documentation

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2,2-bis(chloromethyl)trimethylene bis(bis(2-chloroethyl)phosphate)

EC Number:

253-760-2

EC Name:

2,2-bis(chloromethyl)trimethylene bis(bis(2-chloroethyl)phosphate)

Cas Number:

38051-10-4

Molecular formula:

C13H24Cl6O8P2

IUPAC Name:

2,2-bis(chloromethyl)propane-1,3-diyl tetrakis(2-chloroethyl) bis(phosphate)

Details on test material:

Chemical name: 2,2-bis(chloromethyl)trimethylene bis(bis(2-chloroethyl)phosphate)
Product name: Amgard V6

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

A mixed population of activated sludge micro-organisms obtained from the aeration stage of the Severn Trent Water plc sewage treatment plant at Belper, Derbyshire, UK, treating predominantly domestic sewage

Duration of test (contact time):

28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

20 other: mg C/L

Based on:

DOC

Initial test substance concentration 2

Initial conc.:

79.7 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

TEST CONDITIONS
- Test temperature: 21-22 degree C
- pH: 7.4 (test material and control) 7.5 (standard material)
- Continuous darkness: yes

TEST SYSTEM
- Measuring equipment: Ionic 555 TOC Analyzer

SAMPLING
- Sampling frequency: samples taken on day 1, 2, 3, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 27, 28 and 29 (first vessel), 0, 29 (second vessle)

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

Parameter:

% degradation (CO2 evolution)

Value:

5

Sampling time:

29 d

Details on results:

Assessment of ready biodegradability using the CO2 evolution test (modified Sturm test) - according to OECD guidelines, the chemical could not be considered as ready biodegradable (see table 1). Additional investigational work using the activated sludge respiration inhibition test (OECD guideline no.209) confirmed that the test material did not exhibit any toxic effects on the inoculum at the concentrations employed in the test.

BOD5 / COD results

Results with reference substance:

Sodium benzoate: 97% degradation after 28 days, pass value reached within 10 days. Suitability of test method demonstrated.

Any other information on results incl. tables

Table 5.2.1.1: Cummulative biodegradation (%) of the test and reference substance

	% cummulative biodegradation at sampling time (days)
	1	2	3	6	8	10	18	24	29
Test substance	0	0	0	0	0	1	0	0	5
Reference substance	3	41	54	79	77	82	88	89	97

 

 

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

reference substance (sodium benzoate): 97% degradation after 28 days, pass level reached within 10 days

Interpretation of results:

other: not readily biodegradable

Conclusions:

2,2-bis(chloromethyl)trimethylene bis(bis(2-chloroethyl)phosphate) is not ready biodegradable according to the OECD guidelines (5% degradation after 28 days)

Executive summary:

In a screening test for biodegradation in water (OECD Guideline No 301B) 2,2-bis(chloromethyl)trimethylene bis(bis(2-chloroethyl)phosphate) attained 5% degradation after 28 days. The test substance is therefore considered to be not readily biodegradable, according to terms and conditions of the OECD Guidelines.

An activated sludge respiration inhibition test (OECD guideline no.209) confirmed that the test material did not exhibit any toxic effects on the inoculum at the concentrations employed in the test.