Amines, di-C16-18-alkylmethyl

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

from 1990-06-05 to 1990-06-07

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Analytical monitoring:

no

Details on sampling:

no data

Vehicle:

no

Details on test solutions:

PREPARATION:
Direct dispersion in water. 1.0 g of test substance was dispersed in water and the volume adjusted to 1 litre.
This stock dispersion was then diluted to give a 10 mg/l stock solution from which serial dilutions were made to give the test series.
The test substance was known to absorb to glassware and so saturation of the absorption sites was achieved by soaking the test vessels overnight prior to the start of the test. The test vessels were emptied, rinsed with the solution to be tested and then refilled with the fresh test solution at 0 hours.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: Laboratory culture originating from a strain supplied by I.R.CH.A., France.
- Selection: Gravid adults were isolated 24 hours prior to initiation of the test. Young daphnids produced overnight were used for testing.

ACCLIMATION
- Acclimation period:
- Acclimation conditions: At 21'C in polypropylene vessels containing two litres of dechlorinated and aged tap water.
Culture conditions ensure that reproduction is by parthenogenesis.
- Type and amount of food: mixture of fry fish food (Liquifry) and a suspension of mixed algae (predominantly Chlorell a spp. ) .
- Feeding frequency: daily.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

50 mg/l as CaC03

Test temperature:

22°C

pH:

7.1 - 7.2

Dissolved oxygen:

7.9 - 8.6 mgO2/L

Salinity:

no data

Nominal and measured concentrations:

Nominal concentrations: 0.10, 0.18, 0.32, 0.56, 1.0, 1.8, 3.2, 5.6 and 10 mg/l .

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass beakers each containing 200 ml test solution.
- Aeration: none.
- 9 test concentrations plus 1 control each in duplicate.
- 20 animals per concentration.
- Renewal: no
- Loading: 20 ml test solution per organism.

OTHER TEST CONDITIONS
Photoperiod: 16h light : 8h dark.

TEST MEDIUM / WATER PARAMETERS
- Dechlorinated (with sodium thiosulphate) and aged laboratory tap water.
- Total hardness approximately 50 mg/l as CaC03.

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.45 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL = 0.37 - 0.54 mg/L

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

0.82 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL = 0.64 - 1.1 mg/L

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

0.18 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

NOEC

Effect conc.:

0.18 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

not relevant

Validity criteria fulfilled:

not specified

Executive summary:

Acute toxicity of Amines, bis(hydrogenated tallow alkyl) methyl to Daphnia magna has been investigated through OECD Guideline 202.

Daphnia magna were exposed for 48 hours to a solution of Amines, bis(hydrogenated tallow alkyl) methyl

at different nominal concentrations of 0.10, 0.18, 0.32, 0.56, 1.0, 1.8, 3.2, 5.6 and 10 mg/l.

EC 50 - 48h determined was of 0.45 mg/L and NOEC-48h was of 0.18 mg/L.

Description of key information

Acute toxicity of Amines, bis(hydrogenated tallow alkyl) methyl to Daphnia magna has been investigated through OECD Guideline 202.

There are two acute daphnia tests available evaluated to be of K2 quality. The study from Handly resulted in the lowest EC50 and this result is as a worst-case used as Key result for this endpoint.

Daphnia magna were exposed for 48 hours to a solution of Amines, bis(hydrogenated tallow alkyl) methyl (old CAS no.: 61788-63-4, New CAS no.: 1227096-04-90) at different nominal concentrations of 0.10, 0.18, 0.32, 0.56, 1.0, 1.8, 3.2, 5.6 and 10 mg/L.

The 48h EC50 determined was of 0.45 mg/L.

The 48h NOEC was of 0.18 mg/L.

 

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

0.45 mg/L

Additional information

Four studies have been identified concerning impacts of N-C16-18 (even numbered)-alkyl-N-methyl, C16-18 (even numbered)-alkyl-1-amine on mobility/mortality of invertebrates. None of those studies can be considered as Klimish 1 especially due to analytical measurements which were not done. Nevertheless 2 studies have been identified as sufficiently relevant (Klimish 2) for covering the invertebrate toxicity eventhough analytical measurement was not carried out. They were at least done in accordance with OECD 202 guideline and the test substance was well characterized. The worst-case result has been retained for risk assessment and classification purposes.