Trichromium dicarbide

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro cytogenicity / micronucleus study

Remarks:

Type of genotoxicity: chromosome aberration

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable study report which meets basic scientific principles.

Data source

Reference

Reference Type:

publication

Title:

In vitro genotoxic effects of different combinations of cobalt and metallic carbide particles

Author:

De Boeck, M., N. Lombaert et al.

Year:

2003

Bibliographic source:

Mutagenesis 18(2): 177-186

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

other: OECD 487

Deviations:

not specified

Principles of method if other than guideline:

All details of the method and experimental setting not described in detail.

GLP compliance:

not specified

Type of assay:

in vitro mammalian cell micronucleus test

Test material

Test material information

Constituent 1

Reference substance name:

Trichromium dicarbide

EC Number:

234-576-1

EC Name:

Trichromium dicarbide

Cas Number:

12012-35-0

Molecular formula:

Cr3C2

IUPAC Name:

{[(chromiomethyl)chromio]methylidyne}chromium

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

Cr3C2 chromium carbide
surface area 0.983 m2/g, median diameter 1.2 µm

Method

Species / strain

Species / strain / cell type:

lymphocytes: human PBMC

Metabolic activation:

not specified

Test concentrations with justification for top dose:

10, 50, 100 µg/ml

Vehicle / solvent:

sterile deionized water

Controls

Negative solvent / vehicle controls:

yes

Positive controls:

yes

Positive control substance:

ethylmethanesulphonate

mitomycin C

Details on test system and experimental conditions:

METHOD OF APPLICATION: in medium

DURATION
- Preincubation period: 24 h
- Exposure duration: 48 h

Cytochalasin B was added 44 h after the start of the culture

NUMBER OF REPLICATIONS: dublicates for each concentration

NUMBER OF CELLS EVALUATED:

DETERMINATION OF CYTOTOXICITY
- Method: relative division index

OTHER EXAMINATIONS:
- Comet assay

Evaluation criteria:

Duplicate samples of 1000 cytokinesisblocked (binucleated) lymphocytes were examined for the presence of micronuclei.

For the Comet assay, % of DNA in the tail and tail length were recorded as DNA damage parameters.

Statistics:

Student's t-test for differences between treated and control samples (P<0.0083 for statistical significance). Dose-dependency was tested by a trend test (P<0.0025 for statistical significance).

Results and discussion

Test results

Species / strain:

lymphocytes:

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity

Remarks:

reporting unclear; no statistical analysis on relative division index?

Vehicle controls validity:

valid

Positive controls validity:

valid

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

Trichromium dicarbide at concentrations of 10, 50 and 100 µg/ml did not cause any genotoxicity in human lymphocytes, as measured by the micronucleus test and the Comet assay.

Executive summary:

Exposure of isolated human lymphocytes cells with trichromium dicarbide at concentrations of 10, 50 and 100 µg/ml did not cause any genotoxicity, as measured by the micronucleus test and the Comet assay.