Registration Dossier

Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

Currently viewing:

Administrative data

Link to relevant study record(s)

Description of key information

No toxic effects could be observed in a study with Eisenia fetida.

Key value for chemical safety assessment

Additional information

In a 56-day study, adult earthworms of the species Eisenia fetida were exposed to the test substance in concentrations of 62.5, 125, 250, 500 and 1000 mg/kg soil dry weight, in accordance with the OECD 222 guideline. The mortality and the body weight change of the adult worms were observed and recorded on day 28. The reproduction, number of juveniles per test vessel 56 days after application was observed.

Adult worms:

No mortality of adult worms was observed in the controls and at all replicates with

test substance

NOECMortality: ≥ 1000 mg test substance/kg soil (dw)

LOECMortality: > 1000 mg test substance /kg soil (dw).

NOECBiomass change: ≥ 1000 mg test substance/kg soil (dw)

LOECBiomass change: > 1000 mg test substance /kg soil (dw)

Juvenile worms:

Reproduction by count of juvenile worms:

NOECReproduction: ≥ 1000 mg test substance/kg soil (dw)

LOECReproduction: > 1000 mg test substance/kg soil (dw)

No ECx calculations were performed, because there was no dose response relationship. A significant effect was observed at 500 mg/kg of test substances (Wilcoxon test, one sided -; p ≤0.05). However, this can be disregarded as the highest concentration (1000 mg/kg test substance/kg soil (dw)) and a lower concentration than this (125 mg/kg test substance/kg soil (dw)) showed similar number of offspring which is the range of reproduction in control assays. Therefore, we can conclude that this observation is an outlier.

The results in this study are consistent with all relevant validity criteria and the test is valid according to the test guideline of this study. No deviations from the test guidelines or other incidents occurred during the reported test, which may have influenced the results.