2-chloro-3-(2-chloroethyl)-1,3,2-oxazaphosphinane 2-oxide

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP compliant, guideline study, available as unpublished report, no restrictions, fully adequate for assessment.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)

Deviations:

not specified

GLP compliance:

yes

Remarks:

RCC Umweltchemie AG

Test material

Test material information

Constituent 1

Reference substance name:

Isophamchloride

IUPAC Name:

Isophamchloride

Details on test material:

- Name of test material (as cited in study report): Isophamchloride
- Molecular formula (if other than submission substance): not provided
- Molecular weight (if other than submission substance): not provided
- Smiles notation (if other than submission substance): not provided
- InChl (if other than submission substance): no data
- Structural formula attached as image file (if other than submission substance): no data
- Substance type: no data
- Physical state: White crystals
- Analytical purity: 99.7%
- Impurities (identity and concentrations): no data
- Composition of test material, percentage of components: no data
- Isomers composition: no data
- Lot/batch No.: 023062
- Expiration date of the lot/batch: August 1993
- Stability under test conditions: no data
- Storage condition of test material: Dry and cool

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, domestic (adaptation not specified)

Details on inoculum:

The sludge used for this study was washed three times with tap water and an amount corresponding to 4 g/l dry material was mixed with Sorensen buffer solution pH 7 and the aerated prior to incubation. An amount of 0.5 ml sludge (filetered over cotton-wool) was added to 1000ml test medium.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

135 - <= 137 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

Only stated that the OECD guideline 301E was followed.

Reference substance

Reference substance:

aniline

Results and discussion

% Degradation

Parameter:

% degradation (DOC removal)

Value:

3 - 7

Sampling time:

28 d

BOD5 / COD results

Results with reference substance:

The standard compound (aniline) was readily degraded by 88% during the first 7 days of exposure and at the end of the study (28 days), aniline was degraded by 97%.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

other: slight degradation observed

Conclusions:

Isophamchloride was only slightly degraded by 3 to 7% during the exposure period of 28 days. Therefore this substance is not considered to be readily biodegradable since the pass criteria of 70% DOC removal was not achieved.

Executive summary:

A GLP, OECD 301E ready biodegradation test was conducted on isophamchloride without deviations. The test used aniline as a reference substance which was degraded by 88% after 7 days of exposure. However isophamchloride was only degraded by 3% to 7% at the end of the 28 days exposure period. Therefore isophamchloride is not considered to be ready biodegradable.