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EC number: 235-697-2 | CAS number: 12542-30-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in mammalian cells
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 12 Oct - 5 Dec 1994
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: OECD guideline study conducted in compliance with GLP regulations
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 476 (In Vitro Mammalian Cell Gene Mutation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- testing lab.
- Type of assay:
- mammalian cell gene mutation assay
Test material
- Reference substance name:
- Hexahydro-4,7-methano-1H-indenyl acrylate
- EC Number:
- 235-697-2
- EC Name:
- Hexahydro-4,7-methano-1H-indenyl acrylate
- Cas Number:
- 12542-30-2
- Molecular formula:
- C13 H16 O2
- IUPAC Name:
- 2-Propenoic acid hexahydro-4,7-methano-1H-indenyl ester
- Details on test material:
- - Name of the test substance used in the study report: Dihydrodicyclopentadienylacrylat
- Analytical Purity: 96.8% (sum of 4 isomers; determined by GC-FID)
- Impurities (identity and concentrations):
- 2-ethylhexanol 0.7 area-%,
- acrylic acid 0.06 g/100 g (0.11 g/100 g based on an acid value of 0,86 mg KOH/ g; determined by potentiographic titration),
- water 0.02 g/100 g
- hydroquinone-monomethylether 230 mg/kg.
- Purity test date: 20 Oct 1994; 94L00565
- Lot/batch No.: From vessel No. 27, August 30, 1994 G 403
- Test substance No.: 94/244
Constituent 1
Method
- Target gene:
- HPRT locus
Species / strain
- Species / strain / cell type:
- Chinese hamster Ovary (CHO)
- Metabolic activation:
- with and without
- Metabolic activation system:
- rat liver S-9 fraction
- Test concentrations with justification for top dose:
- 0; 0.0001; 0.0005; 0.001; 0.005; 0.01 mg/mL in experiment 1 without metabolic activation and 0; 0.005; 0.01; 0.05; 0.1; 0.5 mg/mL in experiment 1 with metabolic activation.
0; 0.0005; 0.001; 0.005; 0.0075; 0.01 mg/mL in experiment 2 without metabolic activation and 0; 0.0005; 0.005; 0.05; 0.1; 0.5 mg/mL in experiment 2 with metabolic activation. - Vehicle / solvent:
- DMSO
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: without S-9 mix: Ethylmethanesulfonate; with S-9 mix: 3-Methylcholanthrene
- Details on test system and experimental conditions:
DURATION
- Attachment period: 20-24 h
- Exposure duration: 4 h
- Expression time (cells in growth medium): 1 week
- Selection time (if incubation with a selection agent): 1 week
- Fixation time (start of exposure up to fixation or harvest of cells): 2 weeks
SELECTION AGENT (mutation assays): 6-Thioguanine
DETERMINATION OF CYTOTOXICITY
- Method:
cloning efficiency (Cytotoxicity 1): 17 - 24 hours after termination of the exposure period approx. 200 cells of each replicate (pooled from 2 flasks) were taken in duplicates, seeded into Ham's F12 medium (25 cm2 flasks) and allowed for colony formation (incubator; 1 week). After the end of the incubation period the colonies were fixed, stained and counted.
cloning efficiency (Cytotoxicity 2):
-Same procedure as "Cytotoxicity 1".
-Timepoint: parallel to selection (7 days after exposure)
Other: examination of precipitation- Evaluation criteria:
- The criteria for a positive response are:
- Increases of the corrected mutation frequencies above the concurrent negative control values and above 15 mutants per 10E +06 clonable cells and/or the evidence of a dose-response relationship in the increase in mutant frequencies.
- Evidence of reproducibility of any increase in mutant frequencies.
Results and discussion
Test results
- Species / strain:
- Chinese hamster Ovary (CHO)
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- other: Reduced cell densities/cloning efficiencies were found at concentrations > 0.005 mg/mL in the experiments without metabolic activation. In the presence of S-9 mix cytotoxicity was observed at 0.5 mg/mL.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Precipitation: Precipitation of test substance after addition to test medium was observed at concentrations >=0.1 mg/mL
RANGE-FINDING/SCREENING STUDIES:
-Treatment with Dihydrodicyclopentadienylacrylat at concentrations ranging from 0.005 to 5 mg/mL (with and without S-9 mix). Cytotoxicity was observed at concentrations >= 0.005 mg/mL in the absence of S9-mix, and at concentrations >=0.5 mg/mL in the presence of metabolic activation. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Table 1: Mutant frequencies-experiment 1 without metabolic activation.
Concentration (mg/ml) |
No. of coloniesa |
Mean mutant frequency (per 106cells) |
|||||||
Not corrected |
Correctedb |
||||||||
0 |
A |
0 |
0 |
0 |
0 |
0 |
0 |
2.22 |
2.73 |
|
B |
0 |
1 |
1 |
2 |
2 |
2 |
||
1% DMSO |
A |
0 |
0 |
0 |
0 |
1 |
1 |
1.95 |
2.15 |
|
B |
0 |
0 |
1 |
1 |
1 |
2 |
||
0.0001 |
A |
0 |
0 |
0 |
0 |
0 |
0 |
0.56 |
0.68 |
|
B |
0 |
0 |
0 |
0 |
1 |
1 |
||
0.0005 |
A |
0 |
0 |
0 |
0 |
0 |
0 |
0.56 |
0.78 |
|
B |
0 |
0 |
0 |
0 |
1 |
1 |
||
0.001 |
A |
0 |
1 |
2 |
3 |
3 |
3 |
5.84 |
7.13 |
|
B |
0 |
1 |
1 |
2 |
2 |
3 |
||
0.005 |
A |
0 |
0 |
0 |
0 |
0 |
0 |
3.06 |
4.01 |
|
B |
1 |
1 |
1 |
2 |
2 |
4 |
||
0.01 |
A |
0 |
0 |
0 |
0 |
0 |
0 |
0.00 |
0.00 |
|
B |
0 |
0 |
0 |
0 |
0 |
0 |
||
EMS 0.3 |
A |
22 |
27 |
27 |
29 |
30 |
40 |
98.06 |
116.56 |
|
B |
20 |
25 |
29 |
30 |
32 |
42 |
||
|
|
|
|
|
|
|
|
|
|
a: No of colonies 7 days after seeding, about 300000 cells/flask into selection medium (6 flasks/replicate) |
|||||||||
b: corrected on the basis of the absolute cloning efficiency at the end of the expression period (cytotoxicity 2). |
Table 2: Mutant frequencies-experiment 1 with metabolic activation.
Concentration (mg/ml) |
No. of coloniesa |
Mean mutant frequency (per 106cells) |
|||||||
Not corrected |
Correctedb |
||||||||
0 |
A |
0 |
0 |
1 |
1 |
2 |
2 |
6.39 |
8.23 |
|
B |
1 |
3 |
3 |
3 |
3 |
4 |
||
1% DMSO |
A |
0 |
0 |
0 |
0 |
1 |
2 |
0.84 |
1.12 |
|
B |
0 |
0 |
0 |
0 |
0 |
0 |
||
0.005 |
A |
0 |
0 |
0 |
0 |
0 |
0 |
0.00 |
0.00 |
|
B |
0 |
0 |
0 |
0 |
0 |
0 |
||
0.01 |
A |
0 |
1 |
1 |
2 |
2 |
2 |
3.33 |
3.95 |
|
B |
0 |
0 |
1 |
1 |
1 |
1 |
||
0.05 |
A |
0 |
0 |
0 |
0 |
0 |
1 |
0.56 |
0.69 |
|
B |
0 |
0 |
0 |
0 |
0 |
1 |
||
0.1 |
A |
0 |
0 |
1 |
1 |
2 |
3 |
3.61 |
4.69 |
|
B |
0 |
0 |
1 |
1 |
2 |
2 |
||
0.5 |
A |
0 |
0 |
0 |
0 |
0 |
0 |
3.34 |
4.51 |
|
B |
1 |
1 |
2 |
2 |
2 |
4 |
||
MCA 0.01 |
A |
37 |
40 |
45 |
49 |
50 |
51 |
129.17 |
188.84 |
|
B |
27 |
29 |
30 |
33 |
34 |
40 |
||
|
|
|
|
|
|
|
|
|
|
a: No of colonies 7 days after seeding, about 300000 cells/flask into selection medium (6 flasks/replicate) |
|||||||||
b: corrected on the basis of the absolute cloning efficiency at the end of the expression period (cytotoxicity 2). |
Table 3: Mutant frequencies-experiment 2 without metabolic activation.
Concentration (mg/ml) |
No. of coloniesa |
Mean mutant frequency (per 106cells) |
|||||||
Not corrected |
Correctedb |
||||||||
0 |
A |
0 |
0 |
0 |
1 |
1 |
1 |
2.50 |
3.64 |
|
B |
0 |
0 |
0 |
1 |
2 |
3 |
||
1% DMSO |
A |
0 |
0 |
0 |
0 |
0 |
0 |
1.67 |
2.30 |
|
B |
0 |
0 |
1 |
1 |
1 |
3 |
||
0.0005 |
A |
0 |
0 |
0 |
0 |
0 |
0 |
0.00 |
0.00 |
|
B |
0 |
0 |
0 |
0 |
0 |
0 |
||
0.001 |
A |
0 |
0 |
0 |
0 |
0 |
1 |
3.06 |
4.72 |
|
B |
1 |
1 |
2 |
2 |
2 |
2 |
||
0.005 |
A |
0 |
0 |
0 |
0 |
1 |
1 |
1.67 |
2.5 |
|
B |
0 |
0 |
0 |
1 |
1 |
2 |
||
0.0075 |
A |
0 |
0 |
0 |
0 |
0 |
0 |
2.78 |
4.26 |
|
B |
0 |
1 |
1 |
2 |
2 |
4 |
||
0.01 |
A |
0 |
0 |
0 |
c |
c |
c |
0.00 |
0.00 |
|
B |
0 |
0 |
c |
c |
c |
C |
||
EMS 0.3 |
A |
34 |
37 |
41 |
43 |
50 |
50 |
149.45 |
262.33 |
|
B |
26 |
41 |
44 |
50 |
58 |
64 |
||
|
|
|
|
|
|
|
|
|
|
a: No of colonies 7 days after seeding, about 300000 cells/flask into selection medium (6 flasks/replicate) |
|||||||||
b: corrected on the basis of the absolute cloning efficiency at the end of the expression period (cytotoxicity 2). |
|||||||||
c: not seeded because not enough cells (due to toxicity) |
Table 4: Mutant frequencies-experiment 2 with metabolic activation.
Concentration (mg/ml) |
No. of coloniesa |
Mean mutant frequency (per 106cells) |
|||||||
Not corrected |
Correctedb |
||||||||
0 |
A |
0 |
0 |
0 |
0 |
0 |
1 |
2.78 |
4.22 |
|
B |
0 |
1 |
1 |
1 |
2 |
4 |
||
1% DMSO |
A |
0 |
0 |
0 |
0 |
0 |
0 |
0.00 |
0.00 |
|
B |
0 |
0 |
0 |
0 |
0 |
0 |
||
0.0005 |
A |
1 |
2 |
2 |
3 |
3 |
5 |
5.56 |
9.25 |
|
B |
0 |
0 |
0 |
1 |
1 |
2 |
||
0.005 |
A |
0 |
0 |
0 |
0 |
0 |
1 |
1.12 |
1.74 |
|
B |
0 |
0 |
0 |
1 |
1 |
1 |
||
0.05 |
A |
0 |
0 |
0 |
0 |
0 |
1 |
1.12 |
1.70 |
|
B |
0 |
0 |
0 |
1 |
1 |
1 |
||
0.1 |
A |
1 |
1 |
1 |
3 |
3 |
5 |
5.28 |
8.01 |
|
B |
0 |
0 |
1 |
1 |
1 |
2 |
||
0.5 |
A |
0 |
c |
c |
c |
c |
c |
0.00 |
0.00 |
|
B |
0 |
c |
c |
c |
c |
c |
||
MCA 0.01 |
A |
2 |
5 |
6 |
7 |
9 |
13 |
27.78 |
43.53 |
|
B |
7 |
9 |
9 |
9 |
11 |
13 |
||
|
|
|
|
|
|
|
|
|
|
a: No of colonies 7 days after seeding, about 300000 cells/flask into selection medium (6 flasks/replicate) |
|||||||||
b: corrected on the basis of the absolute cloning efficiency at the end of the expression period (cytotoxicity 2). |
|||||||||
c: not seeded because not enough cells (due to toxicity) |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
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