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Environmental fate & pathways

Bioaccumulation: aquatic / sediment

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Administrative data

Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1999-12-17 - 2000-03-02
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2000
Report Date:
2000

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)
Deviations:
yes
Remarks:
Only one test substance concentration (at least two concentrations recommended by OECD 305)
Qualifier:
according to
Guideline:
other: "Method for Testing the Degree of Accumulation of Chemical Substances in Fish Body" stipulated in the "Testing Methods for New Chemical Substances" (July 13, 1974, Revised October 8, 1998, Kanpogyo No.5, Environment Agency, Japan)
GLP compliance:
yes
Remarks:
"OECD Principles of Good Laboratory Practice" (May 12, 1981)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Radiolabelling:
no

Test solutions

Vehicle:
yes

Test organisms

Test organisms (species):
Cyprinus carpio

Study design

Route of exposure:
aqueous
Test type:
flow-through
Water / sediment media type:
natural water: freshwater
Total exposure / uptake duration:
28 d
Total depuration duration:
0 d

Test conditions

Hardness:
Total hardness (Ca, Mg): 132 mg/L
Test temperature:
Test group: 24.8 - 25.8 °C
Control gorup: 25.3 - 25.8 °C
pH:
7.6
Dissolved oxygen:
Ca. 8 mg/L, continuously monitored during the study.
TOC:
1.3 mg/L
Nominal and measured concentrations:
Nominal: 0,1 mg/L
Mean measured: 0.0935 mg/L
Reference substance (positive control):
no

Results and discussion

Lipid contentopen allclose all
Lipid content:
1.69 %
Time point:
start of exposure
Lipid content:
1.96 %
Time point:
end of exposure
Bioaccumulation factor
Type:
BCF
Value:
>= 0 - <= 6.2 dimensionless
Basis:
whole body w.w.
Calculation basis:
steady state
Remarks:
As no time of plateau could be determined, days of analysis 14, 21 and 28 were taken for the calculation of the steady state concentration in the test medium and the relation to the limit of determination in fish gave the upper limit of BCF of 6.2.
Remarks on result:
other: The concentration in none of the 5 treatment samples analyzed during the 28 d period was above the limit of determination. As such, no time of plateau could be determined.
Remarks:
Conc.in environment / dose:0.0935 mg/L
Depuration
Elimination:
not specified
Parameter:
other: The concentration in none of the 5 treatment samples analyzed during the 28 d period was above the limit of determination. As such, no analysis of depuration was possible.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes