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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Administrative data

Endpoint:
toxicity to soil macroorganisms except arthropods: long-term
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2009-11-19 to 2010-01-16
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2010
Report Date:
2010

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 222 (Earthworm Reproduction Test (Eisenia fetida/Eisenia andrei))
Version / remarks:
DIN ISO 11268-2: 1998 (E), BBA Guideline (1994)
Deviations:
yes
Remarks:
Room temperature decreased several times to a minimum of 17 °C for a total time of about 6 hours. The soil moisture of all test vessels deviated by more than 10 % of the initial moisture at the end of the test.
GLP compliance:
yes (incl. certificate)
Remarks:
According to German Chemikaliengesetz and Directive 88/320/EEC

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
no

Test substrate

Vehicle:
yes

Test organisms

Test organisms (species):
Eisenia fetida
Animal group:
annelids

Study design

Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
yes
Total exposure duration:
56 d
Remarks:
Removement of adults after 28 days

Test conditions

Test temperature:
20 +/- 2°C
pH:
Application rate
[mg test item/kg soil dry weight] pH-value
day 0 day 56
Control 6.37 6.88
Solvent Control 6.44 6.94
1000 6.30 6.46
Moisture:
Application rate
[mg test item/kg soil dry weight] Moisture [%] of dry weight

day 0 day 56
Control 28.3 34.8*
Solvent Control 27.5 35.1*
1000 28.4 33.7*
Nominal and measured concentrations:
Nominal application rate: 1000 mg test substance/kg soil dry weight (DW)
Reference substance (positive control):
yes
Remarks:
1.0 - 2.5 - 5.0 mg Carbendazim/ kg soil DW

Results and discussion

Effect concentrations
Duration:
56 d
Dose descriptor:
NOEC
Effect conc.:
1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Remarks:
, mortality, body weight
Reported statistics and error estimates:
t-tests were carried out for the determination of statistically significant differences compared to the control. A Normality Test and an Equal Variance Test were conducted prior to running the t- tests. P-values for both Normality and Equal Variance Test were 0.05. The a-value for ANOVA test (acceptable probability of incorrectly concluding that there is a difference) was a = 0.05.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes