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EC number: 210-856-9 | CAS number: 624-65-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- GLP compliant guideline study, available as unpublished report, minor restriction in design (no E.coli/WP2 strain or S.typhimurium TA102 strain used) and/or reporting but otherwise adequate for assessment; however, no details on purity of the test material are given
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- no E. coli/WP2 strain or S. typhimurium TA102 strain used
- Qualifier:
- according to guideline
- Guideline:
- other: EU Method B.14 (Salmonella typhimurium - reverse mutation assay)
- Qualifier:
- according to guideline
- Guideline:
- other: Environmental Protection Agency, Code of Federal Regulations, Title 40, Subpart F-Genetic Toxicity, Revision July 1, 1986 “The Salmonella Typhimurium Reverse Mutation Assay"
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 3-chloropropyne
- EC Number:
- 210-856-9
- EC Name:
- 3-chloropropyne
- Cas Number:
- 624-65-7
- Molecular formula:
- C3H3Cl
- IUPAC Name:
- 3-chloroprop-1-yne
- Details on test material:
- - Name of the test material (as cited in study report): Propargylchloride
- Batch No.: P8/87 (Lab. - No.54/L)
- Physical state: yellow liquid
- Stability, pure: 6 months
- Stability, in solvent: in water, ethanol, methanol, acetone, DMSO, DMF and Toluene >24 hours
- Storage: room temperature
- Expiration date: 1989-12-01
Constituent 1
Method
- Target gene:
- His-locus
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: TA 1535, TA 1537, TA 1538, TA 98 and TA 100
- Additional strain / cell type characteristics:
- not applicable
- Metabolic activation:
- with and without
- Metabolic activation system:
- Liver S9 fraction of Aroclor - induced rats
- Test concentrations with justification for top dose:
- - without S9 mix: 10.0, 33.3, 100.0, 333.3, 666.6, 1000.0 µg/plate
- with S9 mix: 33.3, 100.0, 333.3, 666.6, 1000.0, 5000.0 µg/plate - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: Toluene
- Justification for choice of solvent/vehicle: It was chosen to its solubility properties and its relative nontoxicity for the bacteria.
Controlsopen allclose all
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Toluene
- True negative controls:
- no
- Positive controls:
- yes
- Remarks:
- 10 µg/plate
- Positive control substance:
- sodium azide
- Remarks:
- TA1535, TA100; without metabolic activation
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Toluene
- True negative controls:
- no
- Positive controls:
- yes
- Remarks:
- 50 µg/plate
- Positive control substance:
- other: 4-nitro-o-phenylene-diamine
- Remarks:
- TA1537, TA1538, TA98; without metabolic activation
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Toluene
- True negative controls:
- no
- Positive controls:
- yes
- Remarks:
- 10 µg/plate
- Positive control substance:
- other: 2-aminoanthracene
- Remarks:
- TA1535, TA1537, TA1538, TA98, TA100; with metabolic activation
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Exposure duration: 72 h
NUMBER OF REPLICATIONS:
- Experiment performed in duplicate
- Three plates per condition per experiment
DETERMINATION OF CYTOTOXICITY:
- Method: other: growth inhibition of revertant clones
DATA RECORDING
- The colonies were counted using the BIOTRAN III counter - Evaluation criteria:
- - A test article is considered as positive if either a significant dose-related increase in the number of revertants or a significant and reproducible increase for at least one test concentration is induced.
- A test article producing neither a significant dose-related increase in the number of revertants nor a significant and reproducible positive response at any one of the test points is considered non-mutagenic in this system.
- A test article is considered as mutagen if in strain TA 100 the number of reversions is at least twice as high and in strains TA 1535, TA 1537, TA 1538, and TA 98 it is at least three times higher as compared to the spontaneous reversion rate.
- Also, a dose-dependent increase in the number of revertants is regarded as an indication of possibly existing mutagenic potential of the test article regardless whether the highest dose induced the above described enhancement factors or not. - Statistics:
- No appropriate statistical method was available.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium, other: TA1535, TA100
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium, other: TA1537, TA1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- RANGE-FINDING/SCREENING STUDIES:
To evaluate the toxicity of the test article a pre-study was performed with strains TA 98 and TA 100.
The plates with the test article showed normal background growth up to 5000.0 µg/plate in strain TA 98 and TA 100, respectively.
However, no spontaneous revertants occurred at 5000.0 µg/plate in strain TA 98 and at 1000.0 and 5000.0 µg/plate in strain TA 100, all without metabolic activation.
ADDITIONAL INFORMATION ON CYTOTOXICITY:
Toxic effects, evidenced by a reduction in the number of spontaneous revertants, occurred in strain TA 1537 at 100.0 and 333.3 µg/plate without S9 mix and at 5000.0 µg/plate with S9 mix (exp. II). Also in strain TA 1538 at 5000.0 µg/plate with S9 mix (exp. II).
The plates incubated with the test article showed normal background growth up to 1000.0 µg/plate without S9 mix and up to 5000.0 µg/plate in the presence of S9 mix in all strains used.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
positive
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