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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
16 October 1996 to 28 November 1996
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes (incl. QA statement)
Specific details on test material used for the study:
Identity: FAT 40557/A
Batch-no.: PV1
Purity: 65 %
Appearance: Solid powder, red-brown
Solubility (g/l): >100
Expiration date: 30 September, 2002
Storage conditions: At room temperature
Analytical monitoring:
yes
Details on sampling:
All test medium samples for the analytical determinations were taken from the approximate center of the test vessels (after mixing the medium) and kept at -18 °C to -25 °C until analysis.
Vehicle:
yes
Details on test solutions:
100 mg test substance samples was dissolved in 200 mL test medium in a beaker, homogenized for 5 minutes by ultrasonic treatment and then filled up to 1 litre with test water. Test concentrations were prepared from this stock solution.
Test organisms (species):
Daphnia magna
Details on test organisms:
The organism used in this test system is the species Daphnia magna Strauss. The daphnids were bred in the laboratories of the Product Ecology under standardised conditions.

Breeding:
-Cultures of Daphnia magna are held in glass vessels containing approx. 3.0 litre of reconstituted water at 18- 22 °C. The water is renewed partially three times per week. The daphnids are fed with a suspension of green algae (Scenedesmus subspicatus) supplemented by a TETRAMIN-extract in such quantities that the food will be consumed within 24 hours.

Pretreatment:
- 24 hours before the start of the exposure reproductive daphnids were separated from the young daphnids by sieving all individuals through a 1000 µm sieve.
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Hardness:
282 mg/L CaCO3
Test temperature:
21°C
pH:
8
Dissolved oxygen:
97-98 %
Nominal and measured concentrations:
- Nominal: 100 mg/L
- Measured: in the range of 95-96 % of nominal values
Details on test conditions:
The daphnia were exposed to the test substance in 250 mL beakers containing 80-100 mL test medium with 10 animals per beaker. All tests were run in duplicate. The Daphnia were exposed to the test substance in 250 mL beakers containing 80-100 mL test medium. Light regime was 12h light/12h dark.
Reference substance (positive control):
yes
Remarks:
Potassium dichromate
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
No immobilisation was noted in the blank control or the test concentration of 100 mg/L.
Results with reference substance (positive control):
Last reference control of August 29, 1996: EC50 = 1.22 mg/L

Determination of the EC-values:


Due to the results of a pretest the test of FAT 40557/A was performed as limit test with a concentration of 100 mg/l and a blank control without test substance. During the test the actual test concentrations in the analysed test media were in the range of 95 - 96 % of the nominal values. Therefore, the test substance was sufficiently stable and the reported results were related to the nominal values of the test concentrations of the test article. The biological results are summarized in Table 1. No immobilization and abnormal behaviour of the Daphnia was observed in the blank control and in the test concentration of 100 mg/l. Thus, the EC0 value after 48 hours was determined to be 100 mg/l. The EC100 value could not be determined.

Validity criteria fulfilled:
yes
Conclusions:
The 48-h EC50 of FAT 40557/A is >100 mg/L in Daphnia magna.
Executive summary:

The acute toxicity of the test substance to Daphnia magna was determined in a GLP-compliant 48 hours static test according to the OECD Guideline 202. The nominal concentration for the main-test was 100 mg/L (limit test). Analysis of the test media showed that concentrations maintained within 95 -96 % of the nominal value. No immobilization and abnormal behaviour of the Daphnia was observed in the the controls and test medium throughout the test. Due to absence of effects, the 48-h EC50 value could not be calculated, but was clearly higher than 100 mg/L.

Description of key information

The 48-h EC50 of test substance is >100 mg/L in Daphnia magna.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Dose descriptor:
EC50
Effect concentration:
> 100 mg/L

Additional information

The acute toxicity of the test substance to Daphnia magna was determined in a GLP-compliant 48 hours static test according to the OECD Guideline 202. The nominal concentration for the main-test was 100 mg/L (limit test). Analysis of the test media showed that concentrations maintained within 95 -96 % of the nominal value. No immobilization and abnormal behaviour of the daphnids was observed in the controls and test medium throughout the test. Due to absence of effects, the 48-h EC50 value could not be calculated, but was clearly higher than 100 mg/L.