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EC number: 464-080-2 | CAS number: 613246-79-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2004-10-06 to 2005-08-17
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- adopted 2004-04-13
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- cited as 92/69/EWG method C.2 as of 1992-07-31
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
As the test item is poorly water soluble, the "water-accommodated fraction" (WAF) was used in the first experiment. This was done by weighing of the nominal load 100 mg/L, adding the corresponding amount of dilution water and shaking vigorously for 24 hours. The resulting solution was filtrated through 0.45 µm filters.
In the second experiment, the WAF was prepared by weighing of the nominal load 100 mg/L, adding the corresponding amount of dilution water and stirring for 24 hours in¬stead of shaking. The resulting solution was left to stand for 15 minutes, then the lower phase was taken for the test. The TOC of the test solution was measured after 30 minutes of stirring and after the stirring period of 24 hours. The TOC lay in a range between 0 and 2.2 mg/L. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain: Berlin
- Source: Umweltbundesamt Berlin
- Age at study initiation: between 0 and 24 hours
- Food type: green algae (Desmodesmus subspicatus)
- Feeding during test. none
- Lighting: none
24 hours before the start of the test, the adult animals were separated from the young. 23 hours later, the adults were caught with the help of a glass tube, and the newborn daph-nia, aged between 0 and 23 hours, were sieved from the medium and immediately placed into a 250 mL-beaker containing dilution water. After a settling-in period of 30 minutes, animals which showed no apparent damage were used for the test.
Daphnia magna is bred in the LAUS GmbH throughout the year. The animals are kept for the use in toxicity tests. They multiply by parthenogenesis, thus being genetically identical. The holding is performed similar to the method described in EN ISO 6341. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 250 mg/L
- Test temperature:
- 21.2 - 22.0 °C
- pH:
- 7.8 to 8.2
- Dissolved oxygen:
- The concentration of dissolved oxygen stayed above 7.6 mg/L throughout the test.
- Nominal and measured concentrations:
- As solubility lies below 100 mg/L, the water-accommodated fraction was prepared for the test. This was done by weighing of the nominal load of 100 mg/L, adding the corresponding amount of dilution water and shaking vigorously for 24 hours. The resulting solution was filtrated through 0.45 µm filter.
4.6 / 10 / 22 / 46 / 100 / 220 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers, nominal volume 2 L; The beakers used are made of glass. They were cleaned by scrubbing with detergent, multiple rinsing with drinking water, rinsing with diluted hydrochloric acid and finally rinsing with deionized water. They were then dried at 105 °C.
- Renewal rate of test solution : after 24 hours
- No. of organisms per vessel: 5
- No. of vessels per concentration: 4
- No. of vessels per control: 4
The water-accommodated fraction was prepared for the test. This was done by weighing of each nominal load, adding the corresponding amount of dilution water and stirring slowly for 24 hours. The solutions were left to stand for about 15 minutes. Then the lower phase was used for the test.
Preparation of the Test Solutions
A stock solution containing 250 mg K2Cr2O7 /L in deionized water was diluted with dilution water to give a series of concentrations ranging from 0.625 to 3.0 mg/L. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (CAS 7778-50-9)
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 52 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 13 - 320 mg/L
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 4.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 27 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 4.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- First Experiment
Immobilities
In the control, none of the daphnia died or showed any signs of abnormal behaviour throughout the test. As all daphnia in the treatment were immobilised on the surface after 24 hours, the test was terminated.
pH and O2, Temperature
The pH values in the test media and the control ranged from 7.8 to 8.2. The concentration of dissolved oxygen stayed above 8.4 mg/L throughout the test. Temperature range was 19.8 - 20.7 °C.
Assessment
Immobilisation of the daphnia was probably caused by the oily film which could be seen on the surface. During the shaking period for the preparation of the WAF, an emulsion was produced. This emulsion couldn't be separated by membrane filtration and during the test, the unsolved part of the test item rose to the surface. The effect on the daphnia can be stated as a physical effect. Therefore the experiment was repeated, but the test solution was prepared by stirring instead of shaking.
Second Experiment
Immobilities
In the control, none of the daphnia died or showed any signs of abnormal behaviour throughout the test.
pH and O2 , Temperature
The pH values in the test media and the control ranged from 7.8 to 8.2. The concentration of dissolved oxygen stayed above 8.0 mg/L throughout the test. Temperature range was 20.9 - 21.5 °C.
TOC-Determination
The TOC of the test solutions was measured 30 minutes after begin of the stirring period and after 24 hours of stirring. Th TOC concentration was determined to be 2.2 mg/L after 24h.
The daphnia were immobilised caused by a thin oily film on the surface. Concerning the observed immobility values, a difference could be noticed between 24 and 48 hours. After 24 hours the medium was renewed. Based on these results, the third experiment was performed under the same conditions with six concentrations in a geometric series.
Third Experiment
Immobilities
In the control, none of the daphnia died or showed any signs of abnormal behaviour throughout the test.
pH and O2, Temperature
The pH values in the test media and the control ranged from 7.8 to 8.2. The concentration of dissolved oxygen stayed above 7.6 mg/L throughout the test. Temperature range was 21.2 - 22.0 °C.
Assessment
In the treatments, a thin oily film on the surface could be observed. This film was probably responsible for the immobilisation. As a dose-response correlation is visible, this test was used to determine the biological results. - Results with reference substance (positive control):
- The pH values in the test media and the control ranged from 7.9 to 8.2. The concentration of dissolved oxygen stayed above 8.1 mg/L throughout the test. Temperature range was 21.0 - 21.1 °C.
The 24h-EC50 of K2Cr2O7 should lie between 0.6 and 2.1 mg/L. The 24h-EC50i of K2Cr2O7 was determined as 1.9 mg/L.
Immobilisation in the controls may not exceed 10 %. Immobilisation in the controls was 0 %
The concentration of dissolved oxygen at the end of the test must be at least 3 mg/L . The lowest concentration of dissolved oxygen at the end of the test was 7.6 mg/L.
Thus, all validity criteria were met. - Reported statistics and error estimates:
- The estimation of the 24h EC50 of the test item was accomplished using the software Origin.The data were evaluated using sigmoidal fit. Since there were only two values between 0 % and 100 % immobility, a linear fit with a probability-y-axis could not be performed.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 (48 h) of Sika Hardener LTJ was calculated to be 27 mg/L. The highest concentration causing no immobility (no observed effect concentration, NOEC) after 48 hours amounted to 4.6 mg/L. The EC100 was 100 mg/L, which was observed after 48 hours.
- Executive summary:
The acute toxicity of the Water Accommodated Fraction (WAF) of Sika Hardener LTJ to Daphnia magna (water flea) was tested under semi-static conditions for 48 hours in accordance with OECD guideline no. 202 and EU method C.2. The test item is immiscible with water and therefore a WAF was prepared. Due to the hydrolysis of SIKA Hardener LTJ no analytial method for the determination of the concentration was available. Thus, the nominal concentrations was used for calculation. The EC50 (48 h) was calculated to be 27 mg/L. The highest concentration causing no immobility (no observed effect concentration, NOEC) after 48 hours amounted to 4.6 mg/L. The EC100 was 100 mg/L, which was observed after 48 hours. All validation criteria were fullfilled.
Reference
Immobilities
Nominal Concentration in mg/L | Immobility 24 hours | Immobility 48 hours | ||||||
abs. | in % | abs. | in % | |||||
0 | 0/5 0/5 | 0/5 | 0/5 | 0 | 0/5 0/5 | 0/5 | 0/5 | 0 |
4.6 | 0/5 0/5 | 0/5 | 0/5 | 0 | 0/5 0/5 | 0/5 | 1/5 | 5 |
10 | 1/5 1/5 | 1/5 | 2/5 | 25 | 5/5 5/5 | 5/5 | 5/5 | 100 |
22 | 1/5 0/5 | 2/5 | 0/5 | 15 | 2/5 3/5 | 3/5 | 0/5 | 40 |
46 | 2/5 2/5 | 3/5 | 2/5 | 45 | 3/5 3/5 | 5/5 | 2/5 | 65 |
100 | 4/5 3/5 | 5/5 | 3/5 | 75 | 5/5 5/5 | 5/5 | 5/5 | 100 |
220 | 5/5 4/5 | 4/5 | 3/5 | 80 | 5/5 4/5 | 5/5 | 5/5 | 95 |
pH and O2, Temperature
Nominal Concentration in mg/L | pH | O2-Concentration in mg/L | ||||||
0 h | 24 h | 48 h | 0 h | 24 h | 48 h | |||
new | old | new | old | new | old | new | old | |
4.6 | 8.1 | 8.1 | 7.8 | 8.1 | 7.9 | 8.1 | 7.9 | 8.0 |
10 | 8.0 | 8.1 | 7.8 | 8.2 | 7.7 | 8.1 | 7.8 | 8.0 |
22 | 8.0 | 8.1 | 7.9 | 8.2 | 7.7 | 8.1 | 7.6 | 8.0 |
46 | 7.9 | 8.1 | 7.9 | 8.2 | 7.6 | 8.1 | 7.7 | 8.0 |
100 | 7.9 | 8.2 | 7.8 | 8.2 | 7.6 | 8.1 | 7.8 | 8.0 |
220 | 7.9 | 8.1 | 7.9 | 8.2 | 7.6 | 8.1 | 7.6 | 7.7 |
Description of key information
The acute toxicity of the test substance to Daphnia magna was tested under semi-static conditions according to OECD guideline 202. The EC50 (48 h) was calculated to be 27 mg/L. The NOEC was calculated to be 4.6 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 27 mg/L
Additional information
The acute toxicity of the Water Accommodated Fraction (WAF) of Sika Hardener LTJ to Daphnia magna (water flea) was tested under semi-static conditions for 48 hours in accordance with OECD guideline no. 202 and EU method C.2. The test item is immiscible with water and therefore a WAF was prepared. Due to the hydrolysis of SIKA Hardener LTJ no analytial method for the determination of the concentration was available. Thus, the nominal concentrations was used for calculation. The EC50 (48 h) was calculated to be 27 mg/L. The highest concentration causing no immobility (no observed effect concentration, NOEC) after 48 hours amounted to 4.6 mg/L. The EC100 was 100 mg/L, which was observed after 48 hours. All validation criteria were fullfilled.
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