Sodium 1-amino-9,10-dihydro-9,10-dioxo-4-[[3-[(1-oxopropyl)amino]phenyl]amino]anthracene-2-sulphonate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1980

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

not specified

Qualifier:

according to guideline

Guideline:

other: AFNOR NF T-90 301 (Avril 1974)

Deviations:

not specified

GLP compliance:

no

Specific details on test material used for the study:

None

Analytical monitoring:

no

Details on sampling:

None

Vehicle:

no

Details on test solutions:

None

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna straus
- Source: I.R.CH.A
- Length at study initiation: 560-800 micrometer

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

24 h

Post exposure observation period:

None

Hardness:

N/A

Test temperature:

20 ± 2 °C

pH:

8.41

Dissolved oxygen:

6.5 mg O2/L

Salinity:

freshwater used

Nominal and measured concentrations:

Nominal concentrations:
- Preliminary test: 8, 10, 13, 17, 22, 29 mg/L.
- Main test: 17, 22, 29, 37, 48 mg/L.

Details on test conditions:

TEST SYSTEM:
- No. of organisms per vessel: 5 daphnids
- No. of vessels per concentration (replicates): 4 replicates
- No. of vessels per control (replicates): 4 replicates
- No. of vessels per vehicle control (replicates): No data


TEST MEDIUM / WATER PARAMETERS
preparation of dilution water:
- NaHCO3 : 0.200 g
- CaCl2 or CaCl2 H2O: 0.224 g
- K2SO4: 0.026 g
- Bidistilled water until 1000 ml

Dissolved oxygen in dilution water should be greater than 80%. If it is not the case, aerate the solution until the concentration of dissolved oxygen is achieved.


OTHER TEST CONDITIONS
- Adjustment of pH: pH should be at 8.0 ± 0.2. If not, the pH will be adjusted using Chloridic acid or Sodium hydroxide.

Reference substance (positive control):

yes

Remarks:

Potassium dichromate (K2Cr2O7)

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 17.2 - < 33 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 22.4 - < 27.3

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: corrected value

Details on results:

- Behavioural abnormalities: No
- Observations on body length and weight: No
- Other biological observations: No
- Mortality of control: 0% immobility in daphnia control.
- Other adverse effects control: No
- Abnormal responses: No
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No

Results with reference substance (positive control):

IC50 (24 hours) with Potassium dichromate is determined to be: 0.92-1.45 mg/l.
In general the IC50 for 24 h of the reference substance is around 1.2 mg/L.

Reported statistics and error estimates:

None

None

Validity criteria fulfilled:

yes

Conclusions:

The IC50 of FAT 20242/B in this acute immobilisation test (24 hours exposure time), was determined to be between 17.2- 33 mg/L.
The corrected value of IC50 (exposure time 24 hours) was found to be 22.4 - 27.3 mg/l thus 25 mg/L.

Executive summary:

FAT 20242/B was tested to determine the 24 hr IC50 to Daphnia magna straus. The experiment was performed according to the AFNOR NF T-90 301 (April 1974) guideline, (Determination of the mobility inhibition to Daphnia magna straus).

A preliminary experiment was performed to determine the concentration range for the main test. Nominal concentrations:

- Preliminary test: 8,10, 13, 17, 22, 29 mg/L.

- Main test: 17, 22, 29, 37, 48 mg/L.

In parallel, the reference substance, Potassium dichromate (K2Cr2O7) was also tested to Daphnia magna.

IC50 (24 hours) with Potassium dichromate is determined to be: 0.92 - 1.45 mg/l.

The IC50 of FAT 20242/B in this acute immobilisation test (24 hours exposure time), was determined to be between 17.2 - 33 mg/L.

The corrected value of IC 50 (exposure time 24 hours) was found to be 22.4 - 27.3 mg/l thus 25 mg/L.

Description of key information

The EC50 of FAT 20242/B in this acute immobilisation test (24 hours exposure time), was determined to be between 17.2- 33 mg/L. The corrected value of EC50 (exposure time 24 hours) was found to be 22.4-27.3 mg/l thus 25 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

25 mg/L

Additional information

A key study on FAT 20242/B was conducted to determine the 24 hr IC50 value to Daphnia magna. The experiment was performed according to the AFNOR NF T-90 301 (avril 1974) guideline, (Determination of the mobility inhibition to Daphnia magna straus). A preliminary experiment was performed to determine the concentration range for the main test. In the main study the concentrations tested were 17, 22, 29, 37, 48 mg/L.

In parallel, the reference substance, Potassium dichromate (K2Cr2O7) was also tested to Daphnia magna. IC50 (24 hours) with Potassium dichromate was determined to be: 0.92-1.45 mg/l.

The IC50 of FAT 20242/B in this acute immobilisation test (24 hours exposure time), was determined to be between 17.2- 33 mg/L.

The corrected value of IC 50 (exposure time 24 hours) was found to be 22.4-27.3 mg/l thus 25 mg/L.