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EC number: 293-106-3 | CAS number: 91051-53-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2010-07-01
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study without restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- , 1984
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- Version / remarks:
- , 2007
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Fatty acids, safflower-oil, Et esters
- EC Number:
- 293-106-3
- EC Name:
- Fatty acids, safflower-oil, Et esters
- Cas Number:
- 91051-53-5
- IUPAC Name:
- Fatty acids, safflower-oil, Et esters
- Details on test material:
- - Name of test material (as cited in study report): C-SAT 100042
- Analytical purity: 75 % ethyl linoleate
- Lot/batch No.: S300660001
- Physical state: hazy yellow liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION:
Since the test substance was hardly soluble in water test concentrations were prepared separately in Milli-RO water. Two 1-litre test bottles were filled with 250 mL of test substance mixtures with initial loading rates of 200 mg/L. These mixtures were stirred in closed dark brown glass bottles for 25 hours. Following overnight stirring period the synthetic sewage feed (16 mL) and the activated sludge (200 mL) were added to the test substance mixture. Milli-RO water was added to provide a final volume of 500 mL with a final test substance loading of 100 mg/L. Two controls without test substance were tested in each test series. Optimal contact between the test substance and test medium was ensured applying continuous aeration and stirring during the 3-hour exposure period. Thereafter, oxygen consumption was measured and recorded for approximately 10 minutes.
Test organisms
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Laboratory culture: Activated sludge from the municipal sewage treatment plant “Waterschap As en Maas”, s-Hertogenbosch, The Netherlands, receiving predominantly domestic sewage.
- Preparation of inoculum for exposure: The sludge was coarsely sieved washed and diluted with ISO-medium. A small amount of the sludge was weighed and dried overnight at ca. 150°C to determine the amount of suspended solids (3.8 g/L of sludge as used for the test). The batch of sludge was used one day after collection; 50 mL of a synthetic sewage feed was added per litre of activated sludge at the end of the collection day. The sludge was kept aerated at test temperature until use.
Initial biomass concentration: Number of microorganisms was determined as amount of Mixed Liquor Suspended Solids (MLSS) per litre test medium.
Adjusted ISO medium: formulated using RO-water (tap-water purified by reverse osmosis, GEON Waterbehandeling, Berkel-Enschot, The Netherlands)
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- 18.3 - 18.9 °C
- Nominal and measured concentrations:
- Nominal: 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel (size): 1-litre
- Oxygen bottles (size): approximately 300 mL
- Material, size, headspace, fill volume: glass, 500 mL (fill volume)
- Aeration: no
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- Biomass loading rate: 3.8 g/L activated sludge
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Milli-RO/Milli-Q water (Tap-water purified by reverse osmosis (Milli-RO) and subsequently passed over activated carbon and ion-exchange cartridges (Milli-Q) (Millipore Corp. Bedford Mass. USA)). - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrations
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- The EC50 of 3,5-dichlorophenol is in the range of 5 - 30 mg/L.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
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