Registration Dossier
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EC number: 239-491-3 | CAS number: 15471-17-7
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
Daphnia magna acute toxicity test, OECD 202, EC50 (48h) > 100 mg/L
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
The acute toxicity to aquatic invertebrates of the test substance was tested according to OECD Guideline 202 (EU Method C.2) in a static freshwater test with Daphnia magna as test organisms (Wetton and Mullee, 2001). The study was conducted under certificated GLP compliance. The test duration was 48 hours with a substance concentration of 100 mg/L, which was selected based on the preliminary range-finding study. No reference substance was used since it is not essential according to the guideline description. A temperature of 21 °C was held constant, the pH range was 7.8 - 7.9 for the test compound as well as for the control. The dissolved oxygen range was 8.2 - 8.5 mg O2/L (Control: 8.3 - 8.5 mg O2/L) and an air saturation volume (ASV) of 92 - 96 % (Control: 93 - 96 %) was measured. The reconstituted water had a theoretical hardness of 250 mg/L CaCO3. No immobility of daphnids was detected at the tested concentration, thus as result an EC50 (48h) > 100 mg/L and a NOEC value of 100 mg/L was reported. It was considered unnecessary and unrealistic to test at concentrations in excess of 100 mg/L. According to the results of the experiment, the substance has not to be classified as toxic to aquatic invertebrates regarding to CLP (1272/2008 EU).
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