Serine, 2-[(2,3,4-trihydroxyphenyl)methylene]hydrazide, hydrochloride (1:1)

Water solubility

Administrative data

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: This study was selected as the key study because the information provided for the hazard endpoint is sufficient for the purpose of classification and labelling and/or risk assessment.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2013

Report date:

2013

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Deviations:

no

Remarks:

The study was conducted according to the guideline in effect at the time of study conduct.

GLP compliance:

no

Type of method:

flask method

Test material

Test material information

Constituent 1

Reference substance name:

2-amino-3-hydroxy-N'-[(E)-(2,3,4-trihydroxyphenyl)methylidene]propanehydrazide hydrochloride

EC Number:

940-411-0

Cas Number:

1353749-74-2

Molecular formula:

C10H14ClN3O5

IUPAC Name:

2-amino-3-hydroxy-N'-[(E)-(2,3,4-trihydroxyphenyl)methylidene]propanehydrazide hydrochloride

Details on test material:

- Purity: 99.9%

Results and discussion

Water solubility

Water solubility:

16.3 g/L

Temp.:

20 °C

Remarks on result:

other: range of 16.0 to 16.7 g/L

Any other information on results incl. tables

Solubility Table.

Results	Sample 1	Sample 2	Sample 3
Solubility (g/L)	16.7	16.0	16.1

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information): very soluble (> 10000 mg/L)
This study and the conclusions which are drawn from it fulfil the quality criteria (validity, reliability, repeatability).
Very soluble (average solubility was 16.3 g/L at 20°C)

Executive summary:

The test substance and purified water (10 mL) were weighed upon an analytical balance and added to a 20 mL glass vial. The vial was equipped with a magnetic stirring bar, was then sealed and put in a tempered block set at 20°C. Three replicate samples were prepared containing 500.3, 500.2 and 500.1 mg of the test substance. The blending of the test substance and water resulted in white slurries which turned into clear, light yellow solutions after a couple of minutes of stirring. After approximately one hour new precipitates had formed leaving behind pearly white slurries. The samples were left to stir overnight at 20°C. After that, stirring was stopped and the solids were allowed to settle. From each supernatant was then removed a 5 mL sample using a syringe. The supernatants were filtered via 0.45 µm syringe filters and were collected in pre-weighed round bottomed flasks. The weight of each supernatant was registered. Evaporation resulted in yellow films of solid in the round bottomed flasks (45°C in evaporator water bath). The solids were dried further at 50°C under vacuum overnight and were then weighed. The solubility was then determined.

Average solubility was calculated to be 16.3 g/L (16300 mg/L).