1-methylimidazole

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

pre-GLP, pre-OECD guideline

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1981

Report date:

1981

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Principles of method if other than guideline:

BASF-test (application on intact skin, for 24 hours, under occlusion)

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

1-methylimidazole

EC Number:

210-484-7

EC Name:

1-methylimidazole

Cas Number:

616-47-7

Molecular formula:

C4H6N2

IUPAC Name:

1-methyl-1H-imidazole

Test material form:

liquid

Details on test material:

Substance name: 1-methylimidazol
Substance number: 79/527

Test animals

Species:

rabbit

Strain:

Vienna White

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: M .GAUKLER, 6050 Offenbach
- Weight at study initiation: males 3.1 kg, femalses 3.0 kg
- Diet: Ssniff K, standard diet for rabbits and guinea pigs (Fa. INTERMAST GMBH, Soe) and Ovator Solikanin (Fa. Muskator-Werke, Düsseldorf) ad libitum
- Water: ad libitum

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

TEST SITE
- Area of exposure: 110 cm2 (clipped skin)
- Type of wrap if used: inert foil fixed with a adhesive bandage

REMOVAL OF TEST SUBSTANCE
- Washing: with warm water or a water/Lutrol solution
- Time after start of exposure: 24 h

Duration of exposure:

24 h

Doses:

250, 400 and 640 mg/kg bw

No. of animals per sex per dose:

3 (250 and 640 mg/kg bw) and 6 (400 mg/kg bw)

Control animals:

no

Details on study design:

- Duration of observation period following administration: 15 days
- Frequency of observations and weighing: 1 h and 1, 2, 7 and 15 days
- Necropsy of survivors performed: yes

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

400 - 640 mg/kg bw

Based on:

test mat.

Mortality:

250 mg/kg bw: no mortality occurred
400 mg/kg bw: 2/6 males and 0/6 females
640 mg/kg bw: 1/3 males and 3/3 females

Clinical signs:

other: Resorptive intoxications symptoms: apathy, accelerated breathing, spasms, salivation, excess lacrimation, narrowed pupils Local irritation symptoms: Besides obvious redness of the skin and edema at the end of the observation period necrotic changes in th

Gross pathology:

Animals which died during the study: dilatation of the heart and hyperemia in the lungs. In the animals which were sacrified at the end of the study no effects were observed.

Other findings:

Skin findings: firstly, erythema and edema. During the observation period necrosis was observed.

Applicant's summary and conclusion

Interpretation of results:

Category 3 based on GHS criteria