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EC number: 689-986-7 | CAS number: 1742-79-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 13 May 2013 to 18 March 2014
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Conducted according to current test guidelines and GLP-compliant
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 014
- Report date:
- 2014
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2-oxo-1,2-dihydropyridin-1-yl acetate
- EC Number:
- 689-986-7
- Cas Number:
- 1742-79-6
- Molecular formula:
- C7H7NO3
- IUPAC Name:
- 2-oxo-1,2-dihydropyridin-1-yl acetate
- Reference substance name:
- 1742-78-6
- IUPAC Name:
- 1742-78-6
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- Name: Oxypyrionacetate
CAS number: 1742-78-6
Batch number: 14058400
Purity: 99.96%
Expiry date: 30 November 2013
Storage details: Refrigerated (2 to 8ºC)
Constituent 1
Constituent 2
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0.1, 1.0, 10 and 100 mg/L
At the start of the test (0 hours), duplicate 20 mL samples of freshly prepared test media were taken from the control and the test concentration media preparation flasks for chemical analysis.
At 48 hours, duplicate 20 mL samples were taken for chemical analysis from the pooled expired test media from all test concentrations and control.
Test solutions
- Details on test solutions:
- The dilution water used in the study was ASTM medium. The range-finding / limit test was conducted at nominal test substance concentrations of 0.1, 1.0, 10 and 100 mg/L. A control group containing ASTM medium only was also included.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Juvenile Daphnia magna (Straus), less than 24 hours old, were taken from healthy parental laboratory cultures, held and maintained at the test facility.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Post exposure observation period:
- Not applicable
Test conditions
- Hardness:
- 150 mg/L CaCO3
- Test temperature:
- 18.6 – 18.9
- pH:
- 6.75 to 8.25
- Dissolved oxygen:
- 97-102%
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal concentrations: 0.1, 1.0, 10 and 100 mg/L
Geometric mean measured concentrations: 0.0121, 0.703, 6.86 and 70.1 mg/L - Details on test conditions:
- The test was conducted using static test conditions with no renewal of test media.
The test vessels (nominal volume 150 mL) were glass crystallising dishes with clear lids each containing 100 mL of the appropriate media. Two replicate test vessels were prepared for the 0.1, 1.0 and 10 mg/L concentrations and four replicate test vessels were prepared for the control and 100 mg/L test concentration.
Five juvenile Daphnia magna, less than 24 hours old, were added to each replicate vessel, using a wide bore glass pipette to avoid damaging the animals during transfer. The Daphnia magna were not fed during the test.
After 24 and 48 hours, the Daphnia magna in each test vessel were observed for evidence of immobility. The observations differentiated between mobile and immobile daphnids. An individual was considered immobile if, when the contents of the test vessel were briefly agitated, it did not swim during a 15-second period of observation. In addition, Daphnia magna submerged in the body of the test media and those that were held at the surface of the test media were also recorded.
The test was conducted with a 16-hour light: 8-hour dark photoperiod.
The pH, dissolved oxygen concentration (% air saturation value (ASV) and mg/L) and temperature were determined in freshly prepared test media at the start of the test. Continuous temperatures were measured using a digital (min/max) thermometer in an additional vessel maintained in the study arena.
At the end of the exposure period the test water quality measurements were conducted using pooled replicate samples of old test media at each of the test media concentrations.
Total hardness and alkalinity of the freshly prepared ASTM medium was determined at the start of the test. - Reference substance (positive control):
- not required
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 70.1 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 70.1 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Details on results:
- After 48 hours, the concentration at which no significant (≤10%) immobility occurred was 70.1 mg/L. The NOEC was therefore 70.1 mg/L, relating to the degradation product Oxypyrion.
The 48-hour EC50 value was determined to be greater than 70.1 mg/L, relating to the degradation product Oxypyrion. - Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- Not applicable
Any other information on results incl. tables
Geometric Mean Measured Concentration (mg/L)of |
Number of |
% |
% |
Control |
20 |
0 |
0 |
0.0311 |
10 |
0 |
0 |
0.703 |
10 |
0 |
0 |
6.87 |
10 |
0 |
0 |
70.1 |
20 |
0 |
10 |
|
Geometric Mean Measured Concentration (mg/L) of Degradation Product Oxypyrion |
|
24 hours |
48 hours |
|
EC50 |
>70.1 |
>70.1 |
NOEC |
70.1 |
70.1 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48-hour acute toxicity of Oxypyrionacetate to the freshwater planktonic crustacean, Daphnia magna, was determined in accordance with the requirements of OECD Chemicals Testing Guideline No. 202 Daphnia sp. Acute Immobilisation Test (adopted April 13 2004).
The validity criteria for control immobility (≤10%) and dissolved oxygen (>60% ASV) were both satisfied. The test is therefore considered valid.
As the measured concentrations were outside the range of 80-120% of nominal exposure concentrations of Oxypyrion which is required to report the test concentrations in terms of nominal concentrations, the test concentrations therefore, were reported as geometric mean measured concentrations.
After 48 hours, the concentration at which no significant (≤10%) immobility occurred was 70.1 mg/L. The NOEC was therefore 70.1 mg/L, relating to the degradation product Oxypyrion.
The 48-hour EC50 value was determined to be greater than 70.1 mg/L, relating to the degradation product Oxypyrion. - Executive summary:
The objective of the study was to determine the 48-hour acute toxicity of Oxypyrionacetate to the freshwater planktonic crustacean, Daphnia magna using semi‑static test conditions. The study was conducted in accordance with the requirements of OECD Chemicals Testing Guideline No. 202 Daphnia sp. Acute Immobilisation Test (adopted April 13 2004).
A static, range-finding test was conducted at concentrations 0.1, 1.0, 10 and 100 mg/L; however, due to variable toxicity results, the range finding test was repeated as a range-finding / limit test. The initial range-finding data has been reported at the client’s request.
The static, range-finding / limit test was conducted at concentrations 0.1, 1.0, 10 and 100 mg/L. A control group was also included.
At the start of the test, five juvenile Daphnia magna were added to each test vessel. TheDaphnia magnain each test vessel were observed at 24 and 48 hours. The Daphnia magna were not fed during the test.
The expiry date of the test substance was 30 November 2013; however, the test substance was re-analysed to confirm that the composition had not altered (purity of 99.8%). The test substance was considered acceptable for use on this study.
Initial method development, conducted under Smithers Viscient study 3200294, indicated that at a room temperature of ~20°C, the test substance readily degraded over a period of approximately 3.5 hours. Therefore, due to the test substance rapid degradation it was decided to analyse the degradation product (Oxypyrion) as opposed to the parent compound (Oxypyrionacetate).
Samples of test media were taken for chemical analysis at 0 hour (new test media) and at 48 hours (old test media).
Analysis of the 0 hour samples showed concentrations of Oxypyrion to range between 60% and 74% of nominal concentrations.
Analysis of the old media at 48 hours showed concentrations of Oxypyrion to range between 63% and 71% of nominal concentrations, with the exception of the 0.1 mg/L concentration. The recovery for 0.1 mg/L was significantly reduced (3% of nominal) however, as the NOEC value was 100 mg/L, this anomaly was considered not to impact the test.
As the measured concentrations were outside the range of 80-120% of nominal exposure concentrations of Oxypyrion, which is required to report the test concentrations in terms of nominal concentrations, the test concentrations were therefore reported as geometric mean measured concentrations.
The 24 and 48-hour EC50 values and the corresponding NOEC values were derived empirically from the data and are presented in the table below.
Geometric Mean Measured Concentration (mg/L)
of Degradation Product Oxypyrion24 hours
48 hours
EC50
>70.1
>70.1
NOEC
70.1
70.1
As no significant toxicity was observed during the test it can be empirically derived that the 24-hour toxicity results were greater that the geometric mean measured concentration for the test.
After 48 hours, the concentration at which no significant (≤10%) immobility occurred was 70.1 mg/L. The NOEC was therefore 70.1 mg/L, relating to the degradation product Oxypyrion.
The 48-hour EC50 value was determined to be greater than 70.1 mg/L, relating to the degradation product Oxypyrion.
The validity criteria for control immobility (≤10%) and dissolved oxygen (>60% ASV) were both satisfied. The test is therefore considered valid.
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