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EC number: 689-188-9 | CAS number: 149343-84-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1 September 1993.
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- OECD Guidelines for Testing of Chemicals (1984) No.209 ''Activated Sludge, Respiration Inhibition Test"
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Version / remarks:
- EEC Commission Directive 87/ 302/ EEC.
- Deviations:
- not specified
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- No further details specified in the study report.
- Analytical monitoring:
- no
- Details on sampling:
- The concentration, homogeneity and stability of the test material preparations were not determined by analysis.
- Vehicle:
- no
- Details on test solutions:
- Direct dispersion in water
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Type: A mixed population of activated sewage sludge micro-organisms.
Source: The aeration stage of the Severn Trent Water Plc sewage treatment plant at Belper, Derbyshire, treating predominantly domestic sewage.
Date of collection: 1 September 1993.
Preparation: The sample was maintained at 21 °C with continuous aeration and used on the day of collection.
The pH was equal to 7.3 and the suspended solids equal to 3.7 g/l prior to use. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Post exposure observation period:
- No post exposure observation period specified in the study report.
- Hardness:
- approximately 100 mg/l as CaC03.
- Test temperature:
- 21 °C
- pH:
- 7.3
- Dissolved oxygen:
- Not applicable - respiration inhibition test
- Salinity:
- Not applicable - freshwater study
- Conductivity:
- Not specified
- Nominal and measured concentrations:
- 1000 mg/l nominal - The concentration of the test material preparations was not determined by analysis.
- Details on test conditions:
- Contact time: 30 minutes and 3 hours.
Vessels: 500 ml conical flasks.
Aeration: Compressed air via narrow bore glass tubes.
Measuring apparatus: 250 ml darkened glass BOD bottle fitted with a Yellow Springs BOD probe, (Model 54) and a magnetic stirrer.
Test concentrations: Test material: 1000 mg/1 in triplicate. Reference material: 3.2, 10 and 32 mg/1.
Temperature: 21 °C
Photoperiod: Normal Laboratory lighting.
Control: Inoculated medium without test or reference material.
Procedure: At time "0" 16 ml of synthetic sewage was made up to 300 ml with water in a 500 ml conical flask. 200 ml inoculum were added and the mixture aerated at approximately 0.5-1 litre per minute. Thereafter, at 15 minute intervals the procedure was repeated with appropriate amounts of the test or reference material being added. As each vessel reached 30 minutes contact time the contents were poured into the measuring vessel and the rate of respiration measured. The procedure was repeated after a 3-hour contact period. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol.
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- The rate of respiration for each culture was measured for a 10-minute period (between approximately 8.0 mg O2/l and 1.0 mg O2/l) after 30 minutes and 3 hours contact time.
Percentage inhibition values as calculated from the equation:
% inhibition = [1 – (2RS/{Rc1 + Rc2})] x 100
Where
RS = Oxygen consumption rate for test or reference samples.
Rc1 + Rc2 = Oxygen consumption rates for Controls 1 and 2.
Percentage inhibition is plotted against concentration for the reference material only and the EC50 values derived by inspection for 30 minutes and 3 hours contact time.
JPR Blue 100 EC50 (30 minutes): >1000 mg/l
Variation in respiration rates of Controls 1 and 2: ± 2%
JPR Blue 100 EC50 (3 hours): >1000 mg/L
Variation in respiration rates of Controls 1 and 2: ± 3%
It was considered unnecessary and unrealistic to test at concentrations in excess of 1000 mg/l. - Results with reference substance (positive control):
- 3,5-dichlorophenol EC50 (3 hours): 10 .5 mg/l
- Validity criteria fulfilled:
- not specified
- Conclusions:
- EC50 (3 hours): >1000 mg/l
- Executive summary:
TEST TYPE: Activated sludge, respiration inhibition test.
TEST METHOD: OECD Guideline No 209 and EEC Commission Directive 87/ 302/ EEC.
INOCULUM: Activated sludge micro-organisms.
CONTACT TIME: 30 minutes and 3 hours.
TEMPERATURE: 21 °C
RESULTS
EC50 (30 minutes): >1000 mg/l
EC50 (3 hours): >1000 mg/l
It was considered unnecessary and unrealistic to test at concentrations in excess of 1000 mg/l.
Reference
Oxygen Consumption Rates and Percentage Inhibition for 30 Minutes Contact Time for 3,5-dichlorophenol and JPR Blue 100 in an Activated Sludge Respiration Inhibition Test
Concentration (mg/l) |
O2consumption rates (mg O2/l/min) |
% inhibition |
Control R1 R2 |
0.50 0.52 |
- - |
JPR Blue 100 1000 R1 1000 R2 1000 R3 |
0.52 0.53 0.50 |
<2> <4> 2 |
3,5-dichlorophenol 3.2 10 32 |
0.54 0.29 0.12 |
<6> 43 76 |
<increase>
R1– R3= Replicates 1 – 3
Oxygen Consumption Rates and Percentage Inhibition for 3 Hours Contact Time for 3,5-dichlorophenol and JPR Blue 100 in an Activated Sludge Respiration Inhibition Test
Concentration (mg/l) |
O2consumption rates (mg O2/l/min) |
% inhibition |
Control R1 R2 |
0.44 0.47 |
- - |
JPR Blue 100 1000 R1 1000 R2 1000 R3 |
0.45 0.48 0.44 |
1 <5> 3 |
3,5-dichlorophenol 3.2 10 32 |
0.39 0.22 0.11 |
14 52 76 |
<increase>
R1– R3= Replicates 1 - 3
Description of key information
EC50 (3 hours): >1000 mg/l
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
Additional information
TEST TYPE: Activated sludge, respiration inhibition test.
TEST METHOD: OECD Guideline No 209 and EEC Commission Directive 87/ 302/ EEC.
INOCULUM: Activated sludge micro-organisms.
CONTACT TIME: 30 minutes and 3 hours.
TEMPERATURE: 21 °C
RESULTS
EC50 (30 minutes): >1000 mg/l
EC50 (3 hours): >1000 mg/l
It was considered unnecessary and unrealistic to test at concentrations in excess of 1000 mg/l.
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