Naphthenic acids, reaction products with diethylenetriamine

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

June 1-6, 2009

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Naphthenic acids, reaction products with diethylenetriamine

EC Number:

268-610-1

EC Name:

Naphthenic acids, reaction products with diethylenetriamine

Cas Number:

68131-13-5

Molecular formula:

C16H21N3O2 (minimal)

IUPAC Name:

Naphthenic acids, reaction products with diethylenetriamine

Details on test material:

Test substance: MK195K
Stability: No particular stability concerns
Solubility: Insoluble
Odor: Aromatic
Storage: room temperature

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Cyprinodon variegatus

Details on test organisms:

Sheepshead minnow

Study design

Test type:

static

Water media type:

saltwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Test temperature:

18.5 to 22.2°C

pH:

7.9

Dissolved oxygen:

5.3-7.2 mg/L

Salinity:

31/32 parts per thousand

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

21.1

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95%CL (18.5-24.1mg/L)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

MK195K was insoluble in seawater, upon addition to seawater in the mixing jars the majority of the material would float or be caught up in the stirring vortex. The 96 hour LC50 and 95% confidence limits for MK195K to the sheepshead minnow were determined to be 21.1 (18.5 -24.1) mg/L. The 96 hour maximum concentration causing 0% mortality was determined to be less than 8mg/L.; the minimum concentration causing 100% mortality was 32mg/L. The 96 hour NOEC is 8mg/L.

Applicant's summary and conclusion

Conclusions:

MK195K was insoluble in seawater, upon addition to seawater in the mixing jars the majority of the material would float or be caught up in the stirring vortex. The 96 hour LC50 and 95% confidence limits for MK195K to the sheepshead minnow were determined to be 21.1 (18.5 -24.1) mg/L. The 96 hour maximum concentration causing 0% mortality was determined to be less than 8mg/L.; the minimum concentration causing 100% mortality was 32mg/L. The 96 hour NOEC is 8mg/L.