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EC number: 431-060-1 | CAS number: 153719-38-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1996-01-03 until 1996-04-18
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline-conform study under GLP without deviations.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- -
- EC Number:
- 431-060-1
- EC Name:
- -
- Cas Number:
- 153719-38-1
- Molecular formula:
- Hill formula: C4H8N4O3 CAS formula: C4H8N4O3
- IUPAC Name:
- 3-methyl-N-nitro-3,6-dihydro-2H-1,3,5-oxadiazin-4-amine
- Details on test material:
- - Name of test material (as cited in study report): CA 2343 A (Intermediate of CGA 293343)
- Substance type: mono-constituent organic substance
- Physical state: solid, fine, white powder
- Analytical purity: 96.7 %
- Impurities (identity and concentrations): no data
- Purity test date: no data
- Lot/batch No.: P.503005
- Expiration date of the lot/batch: Date of reanalysis: December 1996
- Stability under test conditions: not reported
- Storage condition of test material: 0 - 5 °C
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
not applicable
Sampling and analysis
- Analytical monitoring:
- not required
- Details on sampling:
- - Concentrations:
- Sampling method: direct measurement of oxygen consumption per hour in mg/liter with a ORION - Electrode Type 97-08, ORION Microprocessor Ionalizer 901 and dotted on a recorder.
- Sample storage conditions before analysis: not applicable
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The preparation was carried out according to the method described in the guideline.
- Eluate: not applicable
- Differential loading: not applicable
- Controls: blank nutrient solution with inoculum (=100% respiratory activity)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): not applicable
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): not applicable
- Evidence of undissolved material (e.g. precipitate, surface film, etc): none reported
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture: not applicable
- Method of cultivation: On return to the laboratory, the sludge will be washed three times by settling for a short period, e.g. 15-30 minutes, decanting the upper layer and adding the same amount of water as the decanted water. The sludge is kept aerated for use during the day. Before use the pH is
checked and adjusted.
- Preparation of inoculum for exposure: The preparation was carried out according to the method described in the guideline. The sludge was separated from the aqueous layer by settling instead of centrifugation. The pH of the sludge before use was 7.4.
- Pretreatment: If the sludge cannot be used on the day of collection. 100 ml synthetic sewage is added to each litre of the activated sludge prepared as described above; this is then aerated overnight at 20 ± 2 °C.
- Initial biomass concentration: The sludge concentration in the test bottles was 1.59 g/l (dry weight).
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 3 h
- Post exposure observation period:
- none reported
Test conditions
- Hardness:
- not reported, however detailed composition of nutrient solution in dechlorinated drinking water is stated in the study report
- Test temperature:
- 20 ± 2 °C
- pH:
- 7.4
- Dissolved oxygen:
- not reported
- Salinity:
- not reported, however detailed composition of nutrient solution in dechlorinated drinking water is stated in the study report
- Nominal and measured concentrations:
- - nominal test item concentrations: 1, 3.2, 10, 32, and 100 mg/l
- nominal reference substance concentrations: 3.2, 10, and 32 mg/l - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): BOD flasks with gas inlet
- Material, size, headspace, fill volume: glass, size 250 ml, headspace 50 ml, fill volume 200 ml
- Aeration: stirring
- Type of flow-through (e.g. peristaltic or proportional diluter): no flow-through design applied
- Renewal rate of test solution (frequency/flow rate): not applicable
- No. of organisms per vessel: not applicable
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- No. of vessels per vehicle control (replicates): 2
- Biomass loading rate: 1.59 g/l
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated drinkîng water
- Total organic carbon: no data (nutrient solution with 16 g/l peptone and 11 g/l meat extract)
- Particulate matter: activated sludge loading 1.59 g/l
- Metals: Na, K, Mg, Ca
- Pesticides: not reported
- Chlorine: dechlorinated drinking water used
- Alkalinity: not reported, but detailed composition of nutirent solution in drinking water stated in the study report
- Ca/Mg ratio: not reported for the drinking water; nutrient solution composition Ca:Mg = 3.4:1
- Conductivity: not reported
- Culture medium different from test medium: no
- Intervals of water quality measurement: at start and at end of test
OTHER TEST CONDITIONS
- Adjustment of pH: yes, from 7.8 to 7.4
- Photoperiod: not applicable
- Light intensity: not applicable
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : oxygen consumption continuously measured via electrode
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3.3
- Justification for using less concentrations than requested by guideline: not applicable
- Range finding study
- Test concentrations: not applicable
- Results used to determine the conditions for the definitive study: not applicable - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorphenol
Results and discussion
Effect concentrations
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: none reported
- Effect concentrations exceeding solubility of substance in test medium: none reported - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: If the respiration rates of the two controls are not within 15 per cent of each other or the reference substance is not in the accepted range (5 to 30 mg/l), the test is invalid and must be repeated.
- Other: - Reported statistics and error estimates:
- The inhibitory values were calculated on the basis of the measured time depend oxygen consumption of a Blank and Test solution. The Results ( EC50 Values ) were determined after calculation the linear regression (formula given in the study report).
Any other information on results incl. tables
Table 1: Consumption rate and inhibitory effects of the test substance and reference
Sample | Conc. (mg/l) | Consumption rate (mg/l/h) | Inhibition (%) |
Blank 1 | 0 | 69.8 | -2 |
Blank 2 | 0 | 66.7 | 2 |
Reference 1 | 32 | 10.7 | 84 |
Reference 2 | 10 | 40 | 41 |
Reference 3 | 3.2 | 60 | 12 |
Test conc. 1 | 100 | 56.6 | 17 |
Test conc. 2 | 32 | 58.8 | 14 |
Test conc. 3 | 10 | 57.7 | 15 |
Test conc. 4 | 3.2 | 57.7 | 15 |
Test conc. 5 | 1 | 57.7 | 15 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the result EC 50 (3h) > l00 mg/l, acc. to the criteria set in 67/548/ECC and OECD-GHS, test item CA 2343 A is not toxic to bacteria.
- Executive summary:
This report describes the experimental techniques and the results obtained in this study to determine the inhibitory concentration of the test substance CA 2343 A on the respiratory activity of aerobic waste-water bacteria. The method acc. to OECD Guideline 209 was applied. Based on the result EC 50 (3h) > l00 mg/l, acc. to the criteria set in 67/548/ECC and OECD-GHS, test item CA 2343 A is not toxic to bacteria.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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