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EC number: 700-786-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Water solubility
Administrative data
Link to relevant study record(s)
- Endpoint:
- water solubility
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 2012
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 105 (Water Solubility)
- Deviations:
- no
- GLP compliance:
- no
- Type of method:
- flask method
- Water solubility:
- > 879 g/L
- Temp.:
- 20 °C
- pH:
- >= 4.07 - <= 4.12
- Details on results:
- A preliminary test indicated that the solubility of the water solubility of the test item was higher than 500 g/L at 20 °C. Due to this high solubility, a lower limit definitive test was performed to show that the water solubility of Sopholiance S was higher than about 800 g/L. Five constituents of the test item were quantified: Lactone A, lactone A’, Sophorolipid B, Sophorolipid C and Sophorolipid D. There was no significant change of the measured concentrations after 24, 48 and 72h. Thus the results obtained after 24, 48 and 72h were averaged to give mean measured concentrations of 135g/L (Lactone A), 17.9g/L (Lactone A’), 211 g/L (Sophorolipid B) 124 g/L (Sophorolipid C) and 43.2g/L (Sophorolipid D). Dividing this value by the known % w/w of each constituent in Sopholiance S resulted in water solubility values of 894, 886, 879, 886 and 896 g/L respectively, thus confirming that each constituent was fully solubilised. From the results of this test it was concluded that the solubility of Sopholiance S in water is higher than 879 g/L at 20 ± 1°C.
- Conclusions:
- The solubility of Sopholiance S in water is higher than 879 g/L at 20 ± 1°C.
- Endpoint:
- water solubility
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 105 (Water Solubility)
- GLP compliance:
- yes (incl. QA statement)
- Type of method:
- flask method
- Key result
- Water solubility:
- 1 000 g/L
- Conc. based on:
- test mat.
- Temp.:
- 20 °C
- pH:
- 7
- Details on results:
- A preliminary test was performed at 1000g/L. A sample of 1g of Freeze dried Sopholiance was dissolved in 1.00ml of ultrapure water. After 1h of mixing a visibly clear homogenous solution was obtained. As the results of the preliminary test were conclusive, a main test was not conducted. From the results of the preliminary test it was concluded that the solubility of Freeze dried Sopholiance in water at 20 °C is ≥ 1000 g/l.
- Conclusions:
- The solubility of Freeze dried Sopholiance in ultrapure water was determined in a
preliminary test as described in the OECD Guideline No. 105.
The result is:
Solubility in water: 1000 g/l at 20°C
This result corresponds to the saturation mass concentration of Freeze dried
Sopholiance in ultrapure water at 20°C.
Referenceopen allclose all
Hydrosolubility results
Test solution |
Lactone A mean concentration (g/L) |
Lactone A’ mean concentration (g/L) |
Sophorolipid B mean concentration (g/L) |
Sophorolipid C mean concentration (g/L) |
Sophorolipid D mean concentration (g/L) |
After 24 h A1(pH = 4.12) B1(pH = 4.08) |
135 128 |
18.5 16.8 |
215 206 |
127 118 |
43.7 40.6 |
After 48 h A2(pH = 4.09) B2(pH = 4.07) |
132 133 |
17.4 17.7 |
201 215 |
126 124 |
43.3 43.4 |
After 72 h A3(pH = 4.07) B3(pH = 4.10) |
140 139 |
18.5 18.2 |
215 211 |
127 122 |
44.4 44.0 |
Mean concentration
RSD (%) |
135
3.3 |
17.9
3.8 |
211
2.8 |
124
2.8 |
43.2
3.1 |
% w/w sophorolipid in Sophorolipids water solution* |
15.1 |
2.02 |
24.0 |
14.0 |
4.82 |
Hydrosolubility of Sophorolipids |
894 |
886 |
879 |
886 |
896 |
Description of key information
The water solubility of the registered substance has been determined at 20.0°C in two independent tests. Both studies were performed according to OECD guideline No 105, using the shake flask method. It was concluded that the water solubility of the registered substance was > 879 g/L (Processium 2012, support study, non-GLP) and ≥ 1000 g/L (Dutriez 2019, key study, GLP). The highest value has been selected as the key value.
Key value for chemical safety assessment
- Water solubility:
- 1 000 g/L
- at the temperature of:
- 20 °C
Additional information
In the first study (Processium 2012), a preliminary test indicated that the solubility of the water solubility of the test item was higher than 500 g/L at 20 °C. Due to this high solubility, a lower limit definitive test was performed to show that the hydrosolubility of Sopholiance S was higher than about 800 g/L. Five constituents of the test item were quantified: Lactone A, lactone A’, Sophorolipid B, Sophorolipid C and Sophorolipid D. There was no significant change of the measured concentrations after 24, 48 and 72h. Thus the results obtained after 24, 48 and 72h were averaged to give mean measured concentrations of 135g/L (Lactone A), 17.9g/L (Lactone A’), 211 g/L (Sophorolipid B) 124 g/L (Sophorolipid C) and 43.2g/L (Sophorolipid D). Dividing this value by the known % w/w of each constituent in Sopholiance S resulted in water solubility values of 894, 886, 879, 886 and 896 g/L respectively, thus confirming that each constituent was fully solubilised. From the results of this test it was concluded that the solubility of Sopholiance S in water is higher than 879 g/L at 20 ± 1°C.
In the second study (Dutriez 2019), a preliminary test was performed at 1000g/L. A sample of 1g of Freeze dried Sopholiance was dissolved in 1.00ml of ultrapure water. After 1h of mixing a visibly clear homogenous solution was obtained. As the results of the preliminary test were conclusive, a main test was not conducted. From the results of the preliminary test it was concluded that the solubility of Freeze dried Sopholiance in water at 20 °C is ≥ 1000 g/L.
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