Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 837-106-9 | CAS number: 71472-57-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 13 May 2019 - 04 June 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 6-methyl-3,4-dihydro-2H-1,4-benzoxazine
- EC Number:
- 837-106-9
- Cas Number:
- 71472-57-6
- Molecular formula:
- C9H11NO
- IUPAC Name:
- 6-methyl-3,4-dihydro-2H-1,4-benzoxazine
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Identity: 6-methyl-3,4-dihydro-2H-1,4-benzoxazine
- CAS Number: 71472-57-6
- Batch: 1394901004
- Purity: >99% (by HPLC area % 225 nm bandwidth 50 nm)
- Physical State/Appearance: Light yellow liquid
- Expiry Date: 01 September 2019
- Storage Conditions: Approximately 4 °C in the dark, will not be exposed to the atmosphere
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Sample storage conditions before analysis: frozen
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION:
A nominal amount of test item (20 mg) was dissolved in test water and the volume adjusted to 2 liters to give the 10 mg/L test concentration from which a series of dilutions was made to give further test concentrations of 5.6, 3.2, 1.8 and 1.0 mg/L.
Each prepared concentration was inverted several times to ensure adequate mixing and homogeneity.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Name: Daphnia magna
- Age at study initiation: less than 24 hours old
- Source: In house laboratory
- Food type: mixture of algal suspension (Pseudokirchneriella subcapitata) and GEMMA Micro 300 fish food suspension
- Frequency: daily
Study design
- Test type:
- static
- Water media type:
- other: Reconstituted water (Elendt M4 medium)
- Limit test:
- no
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- N/A
- Post exposure observation period:
- Any immobilization or adverse reactions to exposure were recorded at 24 and 48 hours after the start of exposure.
Test conditions
- Hardness:
- 2.5 mmol/L (= 250 mg/L as CaCO3)
- Test temperature:
- 21 °C to 23 °C
- pH:
- 7.9 ±0.3
- Dissolved oxygen:
- 8.1 - 8.4
- Salinity:
- Not specified
- Conductivity:
- Not specified
- Nominal and measured concentrations:
- Nominal Concentrations:
Range finding Test (mg/L): 0, 0.1, 1.0, 10.0, 100.0.
Definitive Test (mg/L): 0, 1.0, 1.8, 3.2, 5.6 and 10.0
Analysis of the test preparations at 0 and 48 hours showed measured test concentrations to range from 89% to 96% of nominal. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 150 mL glass vessels containing 100 mL Elendt M4 medium
- Type: static, no renewal of test media
- Aeration: No
- Type (delete if not applicable): closed
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- CaCl2.2H2O: 293.8
- MgSO4.7H2O: 123.3
- KCl: 5.8
- NaHCO3: 64.8
- Na2SiO3.9H2O: 10.0
- NaNO3: 0.274
- KH2PO4: 0.143
- K2HPO4: 0.184
- H3BO3: 500
- FeSO4.7H2O: 200
- MnCl2.4H2O: 100
- LiCl: 50
- RbCl: 50
- SrCl2.6H2O: 50
- NaBr: 12.4
- Na2MoO4.2H2O: 25.2
- CuCl2.2H2O: 6.4
- ZnCl2: 6.3
- CoCl2.6H2O: 2.5
- KI: 2.5
- Na2SeO3: 1.0
- NH4VO3: 0.3
- Na2EDTA.2H2O: 2500
- Thiamine HCl: 75
- Cyanocobalamine (B12): 1.0
- Biotin (B6): 0.75
OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 16 hours light and 8 hours darkness cycle with 20 minute dawn and dusk transition periods
- Light intensity: between 200 and 1200 Lux
- Temperature: 18 to 22 °C
- Feeding during test: No
RANGE-FINDING STUDY
- Test concentrations (mg/L): 0.10, 1.0, 10 and 100 - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate at concentrations: 0.32, 0.56, 1.0, 1.8 and 3.2 mg/L
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 3.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 1.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- ca. 3.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Details on results:
- No sub lethal effects of exposure were observed throughout the test
- Results with reference substance (positive control):
- - Results with reference substance valid?
Yes
- Test concentrations (mg/L): 0.32, 0.56, 1.0, 1.8 and 3.2
- Limit test: No
- Resutls:
24 hour: EC50 (mg/L) - 1.1; 95% Confidence Limits (mg/L) - 0.95 - 1.3; NOEC (mg/L) - 0.56; LOEC (mg/L) - 1.0
48 hour: EC50 (mg/L) - 0.8; 95% Confidence Limits (mg/L) - 0.12 - 1.5; NOEC (mg/L) - 0.56; LOEC (mg/L) - 1.0 - Reported statistics and error estimates:
- The EC50 values and associated confidence limits at 24 and 48 hours and the slope of the response curve and its standard error were calculated by Probit analysis using Linear Maximum Likelihood regression.
The Lowest Observed Effect Concentration (LOEC) and the No Observed Effect Concentration (NOEC) at 24 and 48 hours were calculated using the Step-down Cochran-Armitage test.
All results were calculated using the ToxRat Professional computer software package (TOXRAT).
Any other information on results incl. tables
Cumulative Immobilization Data and Observations in the Range‑finding Test
Nominal Concentration |
Observations (Initial Population: 5 Per Replicate) |
|||||||
24 Hours |
48 Hours |
|||||||
Cumulative ImmobilizedDaphnia |
Observations |
Cumulative ImmobilizedDaphnia |
Observations |
|||||
R1 |
R2 |
R1 |
R2 |
R1 |
R2 |
R1 |
R2 |
|
Control |
0 |
0 |
5N |
5 N |
0 |
0 |
5 N |
5 N |
0.10 |
0 |
0 |
5 N |
5 N |
0 |
0 |
5 N |
5 N |
1.0 |
0 |
0 |
5 N |
5 N |
0 |
0 |
5 N |
5 N |
10 |
2 |
2 |
3 N |
3 P & T |
5 |
5 |
AI |
AI |
100 |
5 |
5 |
AI |
AI |
5 |
5 |
AI |
AI |
Cumulative Immobilization Data and Observations in the Definitive Test
Nominal Concentration |
24 Hours |
|||||||||
Cumulative ImmobilizedDaphnia |
Observations |
|||||||||
R1 |
R2 |
R3 |
R4 |
Total |
% |
R1 |
R2 |
R3 |
R4 |
|
Control |
0 |
0 |
0 |
0 |
0 |
0 |
5N |
5 N |
5 N |
5 N |
1.0 |
0 |
0 |
0 |
0 |
0 |
0 |
5 N |
5 N |
5 N |
5 N |
1.8 |
0 |
0 |
0 |
0 |
0 |
0 |
5 N |
5 N |
5 N |
5 N |
3.2 |
0 |
2 |
1 |
1 |
4 |
20 |
5 N |
3 N |
4 N |
4 N |
5.6 |
2 |
4 |
2 |
2 |
10 |
50 |
3 N |
1 N |
3 N |
3 N |
10 |
2 |
1 |
3 |
4 |
10 |
50 |
3 N |
4 N |
2 N |
1 N |
Nominal Concentration |
48 Hours |
|||||||||
Cumulative ImmobilizedDaphnia |
Observations |
|||||||||
R1 |
R2 |
R3 |
R4 |
Total |
% |
R1 |
R2 |
R3 |
R4 |
|
Control |
0 |
0 |
0 |
0 |
0 |
0 |
5 N |
5 N |
5 N |
5 N |
1.0 |
0 |
1 |
0 |
0 |
1 |
5 |
5 N |
4 N |
5 N |
5 N |
1.8 |
0 |
0 |
0 |
0 |
0 |
0 |
5 N |
5 N |
5 N |
5 N |
3.2 |
0 |
2 |
3 |
1 |
6 |
30 |
5 N |
3 N |
2 N |
4 N |
5.6 |
5 |
5 |
4 |
4 |
18 |
90 |
AI |
AI |
1 N |
1 N |
10 |
4 |
4 |
5 |
5 |
18 |
90 |
1 N |
1 N |
AI |
AI |
R =Replicate
N = Normal
P = Pale
T = Trapped at surface
AI = AllDaphniaimmobilizedApplicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Exposure of Daphnia magna to the test item gave the following results at 48-hour:
EC50 (mg/L): 3.9
95% Confidence Limits (mg/L) 2.2 - 6.8
NOEC (mg/L): 1.8
LOEC (mg/L): 3.2 - Executive summary:
Introduction
A study was performed to assess the acute toxicity of the test item to Daphnia magna. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (April 2004) No 202, "Daphniasp., Acute Immobilisation Test" referenced as Method C.2 of Commission Regulation (EC) No. 440/2008.
Methods
Following a preliminary range‑finding test, 20 daphnids (4 replicates of 5 animals) were exposed to aqueous solutions of the test item at nominal concentrations of 1.0, 1.8, 3.2, 5.6 and 10 mg/L for 48 hours at a temperature of 21°C to 23 °C under static test conditions. The number of immobilized Daphnia was recorded after 24 and 48 hours.
Results
Analysis of the test preparations at 0 and 48 hours showed measured test concentrations to range from 89% to 96% of nominal and so the results are based on nominal test concentrations.
Exposure of Daphnia magna to the test item gave the following results:
Time Point
(Hours)EC50
(mg/L)95% Confidence Limits (mg/L)
No Observed Effect Concentration (NOEC)
(mg/L)Lowest Observed Effect Concentration (LOEC)
(mg/L)48
3.9
2.2
-
6.8
1.8
3.2
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.