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EC number: 203-464-4 | CAS number: 107-12-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1993
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with generally accepted scientific standards and described in sufficient detail
Data source
Reference
- Reference Type:
- publication
- Title:
- Acute toxicity of organic solvents on Artemia salina
- Author:
- Barahona-Gomariz, M. V.
Sanz-Barrera, F.
Sánchez-Fortún, S. - Year:
- 1 994
- Bibliographic source:
- Barahona-Gomariz, M. V., Sanz-Barrera, F. & Sánchez-Fortún, S., 1994. Acute toxicity of organic solvents on Artemia salina. Bulletin of Environmental Contamination and Toxicology, 52(5), pp.766–771.
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Determine the short-term toxic effects of acetonitrile on three ages of Artemia salina
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Acetonitrile
- EC Number:
- 200-835-2
- EC Name:
- Acetonitrile
- Cas Number:
- 75-05-8
- Molecular formula:
- C2H3N
- IUPAC Name:
- acetonitrile
Constituent 1
- Specific details on test material used for the study:
- obtained from Sigma Chemical Company (St. Louis, USA)
Sampling and analysis
- Analytical monitoring:
- not specified
Test organisms
- Test organisms (species):
- Artemia salina
- Details on test organisms:
- - Common name: Brine shrimp
- Source: San Francisco Bay Brand, Inc. (Division of Metaframe Co., Menlo Park, CA, USA)
- Age at study initiation (mean and range, SD): nauplius larvae of Artemia salina aged 24, 48 and 72 h.
- Method of breeding: The method of Persoone et al. (1989) was applied and modified. The Artemia Salina were obtained from 100mg eggs and were hydrated in distilled water at 4°C for 12 hr. Washing separated the sinkers from floaters. The cysts that sank were collected on a Buchner funnel and washed with cold distilled water, followed by synthetic seawater prepared by mixing 35 %0 of Synthetica seasalt (Waterlife Research Ltd., England), with distilled and deionized (Milli-Q) water, stirring for 24 hr, with suitable aeration and successive filtration with thick cellulose filters. They were then incubated in a graduated glass cylinder for about 24 hr at 25°C, with continuous side illumination (15-W fluorescent lamp), in 100 mL of synthetic seawater, at pH 8.6, and with a slight aeration maintained by a small tube in contact with the bottom of the cylinder. The nauplii produced were aspired with Pasteur pippettes and transferred to a glass flask containing 200 mL of the synthetic seawater.
Study design
- Test type:
- static
- Water media type:
- saltwater
- Limit test:
- no
- Total exposure duration:
- 24 h
Test conditions
- Details on test conditions:
- TEST SYSTEM: 1 mL of synthetic seawater
- Test vessel: plastic 16 -mm petri dishes
- No. of organisms per vessel: 10 larvae
- No. of vessels per concentration (replicates): 3 - 5
- No. of vessels per control (replicates): 5
REPLICATES
All experiments were repeated five times.
OTHER TEST CONDITIONS
- Light intensity: dark
- Temperature: 25°C
EFFECT PARAMETERS MEASURED : Mortality. Larvae were considered dead if they did not exhibit any internal or external movement during 10 sec of observation.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Remarks:
- 24h larvae
- Effect conc.:
- 641 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Remarks:
- 48-h larvae
- Effect conc.:
- 521 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Remarks:
- 72-h larvae
- Effect conc.:
- 400 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- Artemia salina 24, 48 and 72 h old demonstrated that there is an increase in toxicity of acetonitrile following longer development of Artemia salina. Artemia salina 72 h old individuals were more sensitive towards acetonitrile than Artemia salina 24 h old indiviudals. There is no statistically significant difference between the toxicity of acetonitrile to Artemia salina 24 h and 48 h old, however, there is a statistically significant difference between the toxicity of acetonitrile to Artemia salina 24 h and 72 h old.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The toxicity of acetonitrile to three ages of Artemia salina was determined. 72-h old Artemia saliva (LC50 = 400 mg/L) were more sensitive than 48 h (LC50 = 521 mg/L) and 24 h old Artemia (LC50 = 641 mg/L).
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