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Diss Factsheets
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EC number: 423-860-2 | CAS number: 56309-94-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1997-11-24 till 1998-02-18
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- Adopted 17 July, 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- EEC directive 92/69
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Samples were taken form 0, 18, 56 and 180 mg/L for analysis at start and end of test. 10 mL were taken from the approximate center of the test vessels. All samples were stored in a deep-freez unit until analysis.
- Vehicle:
- no
- Details on test solutions:
- The test substance was soluble at the concentrations tested. Stock solutions of 100 mg/L were used to prepare the lower concentrations. A solution containing 180 mg/L was separately prepared. Solutions were stirred for ten to twenty minutes, in addition with ultra-sonic treatment applied.
- Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- TEST ORGANISM
- Common name: Carp
- Strain: Teleostei, Cyprinidae (Linnaeus, 1758)
- Source: Zodiac, preofacc, De Haar Vissen, L.U. Wageningen, The Netherlands
- Length at study initiation: 2.18 ± 0.13 cm
- Weight at study initiation: 0.38 ± 0.04 g
ACCLIMATION
- Acclimation period: at least 12 days
- Acclimation conditions: same as test conditions
- Type of food during acclimation: Trouvit
- Feeding frequency during acclimation: Daily
- Health during acclimation: mortality was less than 5 %. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 250 mg CaCO3/L
- Test temperature:
- 20.2 - 20.7 °C
- pH:
- 7.6 - 8.8
- Dissolved oxygen:
- 6.2 - 9.4
- Nominal and measured concentrations:
- Nominal concentrations: 18, 32, 56, 100 and 180 mg/L
Mean measured concentrations: 17.15, not measured, 53.6, not measured and 182 mg/L. Details in Table 3 below. - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass vessels
- Material, fill volume: glass, 4L
- Aeration: not in the first 24 h of the test, thereafter test solutions were gently aerated until end of test
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 0.67 g fish/L, i.e. 7 fish per 4 L of test medium
TEST MEDIUM / WATER PARAMETERS
- Preparation of dilution water: ISO medium according to OECD 203
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h photoperiod daily
- Light intensity: not available
EFFECT PARAMETERS MEASURED
at 2.5, 24, 48, 72 and 96 h mortality and other effects on fish were measured. Dead fish were removed when oberved.
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 0.1, 1, 10, 100 mg/L
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- pentachlorophenol (PCP)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 180 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 56 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: swimming capacity
- Details on results:
- - Behavioural abnormalities and mortalities: yes, see Table 1 and Table 2
- Other biological observations: no
- Mortality of control: no
- Other adverse effects control: no
- Abnormal responses: no
- Any observations that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no
Validation criteria:
- No mortality was observed in the control.
- The analytical program provided clear evidence that the actual concentration had been maintained at more than 80 % of the initial concentrations.
- Further, all test conditions remained within the ranges prescribed by the protocol. - Results with reference substance (positive control):
- The LC50 of the reference substance was 0.14 mg/L (95% fiducial limits: 0.13-0.18 mg/L) after 96h. The range of the 96h-LC50 for carp is generally between 0.10 and 0.46 mg/L based on historical data of reference test approximately every 3 month since April 1988. The carp originating from this batch can be considered as sensitive to toxic substances.
- Sublethal observations / clinical signs:
Table 1: Specification of effects observed during the study
Concentration (mg/L)
Time (hour)
Specification of effects
Relative number
0-56
0-96
No abnormalities
7/7
100
2.5
Hypoactive
7/7
24-48
Hypoactive and discolored
7/7
72
Hypoactive
5/7
Hypoactive and discolored
2/7
96
Hypoactive
6/7
Hypoactive and discolored
1/7
180
2.5
Hypoactive
5/7
Immobile
2/7
24
Hypoactive and discolored
5/5
48
Hypoactive and discolored
1/5
Immobile and discolored
4/5
72-96
Hypoactive and discolored
4/5
Immobile and discolored
1/5
Table 2: Incidence and total mortality
Concentration (mg/L)
Initial number of fish
Cumulative mortality
Total Mortality (%)
2.5h
24 h
48 h
72 h
96 h
0 (control)
7
0
0
0
0
0
0
18
7
0
0
0
0
0
0
32
7
0
0
0
0
0
0
56
7
0
0
0
0
0
0
100
7
0
0
0
0
0
0
180
7
0
2
2
2
2
29
Table 3: Concentrations of test item in test medium
Time [hours]
Concentrations [mg/L]
Nominal
Analysed*
Relative to nominal [%]
0
0
n.d.
n.a.
18
18.7
104
56
59
105
180
191
106
96
0
n.d.
n.a.
18
15.6
86
56
48.3
86
180
173
96
*Mean of duplicate analysis. The maximum deviation between the responses was calculated tor each sample and was < 10%.
n.d. not detected
n.a. not applicable
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test substance has no acute toxicity effect on carp under the present conditions of the test up 100 mg/L, LC50>100 mg/L.
- Executive summary:
The acute toxicity of the test item in Carp was performed according to EU Method, Part C.1. and the OECD guideline No. 203. After a range-finding test, a final test was executed with cyprinus carpio exposed to the test item in concentrations of 18, 32, 56, 100 and 180 mg/L in a static system. Seven carp were exposed per concentration and a control. Samples for analysis were taken at the start and the end of the test. Analysis of the samples taken showed that the measured concentrations were in agreement with the nominal concentrations (less than 20% deviation). In the control and the lower concentrations no mortality was observed, only at 180 mg/L two fish died after 24 h (mortality of 29%). Effects on swimming capacity and hypoactivity were recorded at 100 and 180 mg/L. The test item induced no lethal effects in carp at or below 100 mg/L and no sublethal effects at or below 56 mg/L. The 96h-LC50 is > 100 mg/L.
Reference
Description of key information
The test substance has no acute toxicity effect on carp under the present conditions of the test up 100 mg/L, LC50>100 mg/L (reference 6.1.1 -1).
Key value for chemical safety assessment
Additional information
The acute toxicity of the test item in Carp was performed according to EU Method, Part C.1. and the OECD guideline No. 203. After a range-finding test, a final test was executed with cyprinus carpio exposed to the test item in concentrations of 18, 32, 56, 100 and 180 mg/L in a static system. Seven carp were exposed per concentration and a control. Samples for analysis were taken at the start and the end of the test. Analysis of the samples taken showed that the measured concentrations were in agreement with the nominal concentrations (less than 20% deviation). In the control and the lower concentrations no mortality was observed, only at 180 mg/L two fish died after 24 h (mortality of 29%). Effects on swimming capacity and hypoactivity were recorded at 100 and 180 mg/L. The test item induced no lethal effects in carp at or below 100 mg/L and no sublethal effects at or below 56 mg/L. The 96h-LC50 is > 100 mg/L (reference 6.1.1 -1).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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