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EC number: 617-694-1 | CAS number: 85261-19-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- N-methyl-N-[(2S,3R,4R,5R)-2,3,4,5,6-pentahydroxyhexyl]nonanamide
- EC Number:
- 617-694-1
- Cas Number:
- 85261-19-4
- Molecular formula:
- C16H33NO6
- IUPAC Name:
- N-methyl-N-[(2S,3R,4R,5R)-2,3,4,5,6-pentahydroxyhexyl]nonanamide
- Test material form:
- solid
- Details on test material:
- sample no. MTDID 48740
MEGA 9: 98.1 % (GC)
Water content: 0.3%
pH (aqueous solution 1%): 7.5
Expiry Date: 2017-12
Product Nr: MTDID 48740
Batch: 549612
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Moderately hard, reconstituted water was used for cu lturing daphnids and as the
control/dilution water. The control/dilution water for the def initive test was prepared
by adding t he following amounts: 1.1440 g magnesium sulphate anhydrous, 1.3202 g
ca lcium su lphate, 2.1124 g sodium bicarbonate, 0.0881 g potassium chloride, 10 ml of a
4 mg/L so lution of Vitamin B12 and 40 ml of a 1 mg/ L selenium solution {as sodium
selenate solution) to 20 L of deionised water. One batch of daphnia water {August 01,
2017) was used for this test {Appendix I). It was vigorously aerated with filtered oil-free
compressed air passed through a glass Pasteur pipette prior to use.
Water quality measurements were conducted on the cont rol/dilution water prior to use
{Table 6-2). Dissolved oxygen, conductivity, temperature and pH were measured
according to the Work Instruction for Operation and Maintenance of Thermo Orion
A329 Mult iparameter Meter {BBY2 Wl-00026). Alkalinity was analysed according to
Standard Operating Procedure for pH, Conductivity, Salinity and Alkalinity {Total,
Phenolphthalein) in Water {BBY6 SOP-00026). Hardness was measured according to the
Work Instruction for Total Hardness Analysis by Titration {BBY2 Wl-00040). Ca lcium,
magnesium, sodium, and potassium were measured according to the Standard
Operation Procedure for Analysis of Various Sample Types by ICP-OES {BBY7 SOP-
00018). Alka lin ity, hardness, and analyses for ca lcium, magnesium, sodium, and
potassium were not ana lysed according to GLP.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- The daphnia culture was originally obtained from Aquatic BioSystems (Fort Collins, CO,
USA) and was used to initiate the in-house cu lture {Appendix 1). The in-house daphnia
cultures were maintained according to the Work Instruction for Culturing of Daphnia
magna and Reference Toxicant test (BBY2 Wl-00006). Prior to the test, the Daphnia
cultures were fed approximately 4.0 ml of Pseudokirchnerie/la subcapitata (PKS) and 1.0
ml of yeast-cerea l-grass fermented trout chow (YCT) three t imes per week. The
neonates used in the test were fed approximately 4.0 ml of P. subcapitata prior to test
initiation. The PKS and YCT used for feeding were also obta ined f rom Aquatic
BioSystems.
The test was initiated with four replicates per t reatment, where each replicate
contained five <24-hr old Daphnia magna neonates. Each replicate (test vessel)
consisted of 200 ml of test solution in a 250 ml glass beaker covered with Plexiglas. The
test organisms were not fed during the 48-hr exposure period.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 92 mg/L as CaCO3
- Test temperature:
- 20.1-20.4
- pH:
- 7.9-8.1
- Nominal and measured concentrations:
- Nominal 6.25 - 12.5 - 25 - 50 - 100 mg/L
Measured 6.7 - 13.6 - 27.8 - 54.2 - 111.5 mg/L - Reference substance (positive control):
- yes
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48-h EC50 was > 100 mg/L, NOEC was 100 mg/L
- Executive summary:
A sample of MTDID48740was tested to assess its potentialto cause acute
immobilisationinDaphnia magna,using the procedures outlinedinthe Maxxam Study
Planfor TOX1714,whichis based ontheStandard Operating Procedure(SOP)forthe
OECD202:Daphniasp., AcutelmmobilisationTest(BBY2SOP-00017) and the OECD 202
Daphniasp., AcutelmmobilisationTest(OECD, 2004). This 48hour static aquatic
exposuretest was conducted under controlledconditions using <24-hr oldDaphnia
magnaneonatesover a 48-hr exposure period and was carried outusingthefollowing
nominal concentrations: 6.25,12.5,25,50, and100mgTest ltem/Lin addition to a
negative (laboratory) control(0 mg/L).
At test completion, there were no immobilised or deaddaphnidsin the control orinthe
nominal test item concentrations.
Themean measured concentrations of the testitemwere within 80% to120%(106% to
112%)ofthenominal concentrations; therefore, the biologicalendpointwas calculated
usingthenominal concentrations of the testitem.
Basedon thenominalconcentrations of MTDID 48740:
1.The48-h ECso(and 95% confidencelimits)was >100 mg/L(N/A)
2. TheNo ObservedEffectConcentration (NOEC) was100mg/L
3.The LowestObservedEffectConcentration (LOEC) wasnotobserved
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