Quaternary ammonium compounds, bis(hydrogenated tallow alkyl)dimethyl, acetates

Long-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

supporting study

Study period:

Early 1980's

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Results published in a scientific review. and peer reviewed in the European RAR.

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

GLP compliance:

no

Analytical monitoring:

yes

Details on sampling:

The test concentrations of the surfactants during the chronic toxicity studies were confirmed using a recently developed technique. The extracted quaternary ammonium chloride compounds are separated and quantified using HPLC with a conductivity detector.

Test organisms (species):

Daphnia magna

Details on test organisms:

Early instar daphnids (< 24 h old)

Test type:

semi-static

Water media type:

freshwater

Total exposure duration:

21 d

Hardness:

345-363 (White river)

pH:

8.4-8.6 (White river)

Reference substance (positive control):

no

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

0.38 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Remarks:

in river water

Basis for effect:

other: reproduction and mean length

Duration:

21 d

Dose descriptor:

LC50

Effect conc.:

1.72 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Remarks:

in river water

Basis for effect:

reproduction

Remarks on result:

other: 1.31-2.49

Conclusions:

21-Day NOEC = 0.38 mg/L (reproduction)

Executive summary:

The no observed effect concentration (NOEC) for daphnids exposed to 14C-labeled DSDMAC in river water was 0.38 mg/L. Length, total young and mean brood size of adults exposed to mean concentrations of >= 0.76 mg/L DSDMAC were significantly less (p ≤ 0.05) than the same parameters for adults in the controls and in the remaining test waters. For example, total young produced at the 0.76 mg/L concentration was approximately 38% less than that for adults exposed to 0.38 mg/L. Mean brood sizes reflected these differences in young production, generally declining at concentrations > 0.38 mg/L. Reproductive performance was more sensitive than mortality: the 21-d LC50 was 1.72 (95%CI = 1.31-2.49) mg/L. Based on the toxicity data for daphnids in river water, the acute to chronic ratio or application factor is 0.12 (NOEC/LC50=0.38/3.1).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.38 mg/L