Quaternary ammonium compounds, bis(hydrogenated tallow alkyl)dimethyl, acetates

Long-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

fish short-term toxicity test on embryo and sac-fry stages

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

supporting study

Study period:

Early 1980's

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

Results published in a scientific review. and peer reviewed in the European RAR.

Justification for type of information:

The primary component of all substances provide complete coverage of 68334-33-8. 68334-33-8 shares high structural similarity with 61789-80-8, 68783-78-8, 107-64-2, and 107-64-2. As 68334-33-8 is a UVCB its components encapsulate the other substances except for the counter ion (Cl-). In solution, the counter ions will dissociate from the parent structures. Therefore, we are comparing substances of equivalent nature. CAS 107-64-2 represents the C18 boundary of the 61789-80-8. ignoring the salt component CAS 61789-80-8 is equivalent to CAS 68334-33-8. 68783-78-8 is a worst case of both 68334-338 and 61789-80-8 since it is unsaturated and SP2 characterized carbon-carbon bonds are of higher reactivity. The primary component of CAS 112-02-7 is a substructure of all the other substances.

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 210 (Fish, Early-Life Stage Toxicity Test)

GLP compliance:

no

Analytical monitoring:

yes

Vehicle:

yes

Details on test solutions:

The test species were exposed to five test concentrations of DTDMAC dissolved either in isopropanol and well water, or in triethylene glycol and river water. All effect concentrations are based on mean measured levels of the active ingredient.

Test organisms (species):

Pimephales promelas

Details on test organisms:

Eggs obtained from the U.S. Fish and Wildlife Service at Columbia, Missouri

Test type:

not specified

Water media type:

freshwater

Limit test:

no

Total exposure duration:

35 d

Hardness:

28-31 mg/L CaCO3 (Well water)
62 mg/L CaCO3 (River water)

pH:

6.8-7.6 (Well water)
6.4-6.9 (River water)

Nominal and measured concentrations:

Well water: solvent control; 0.006, 0.013, 0.024, 0.053, 0.09 mg/L
River water: solvent control; 0.04, 0.061, 0.13, 0.23, 0.45 mg/L
All effect concentrations are based on mean measured levels of the active ingredient. In well water the measured concentrations were equal to the nominal concentrations whereas in river water measured concentrations averaged 45-67% of the nominal concentrations.

Reference substance (positive control):

not specified

Duration:

35 d

Dose descriptor:

NOEC

Effect conc.:

0.23 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Remarks:

in standard water

Basis for effect:

other: hatchability and mean weight of larvae

Duration:

35 d

Dose descriptor:

NOEC

Effect conc.:

0.053 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Remarks:

in river water

Basis for effect:

other:

Remarks:

survival, length and weight of larvae

Conclusions:

NOEC-35d = 0.23 mg/L (river water)
NOEC-35d = 0.053 mg/L (well water)

Executive summary:

To assess the long-term toxicity of DTDMAC embryo larval tests were conducted with Pimephales promelas in filtered well water and natural river water. Exposure was initiated within 48 hours after fertilization and continued through 30 days post hatch in a flow-through system. In well water the most sensitive parameters were mean percent survival, length and weight of larvae. The NOEC was 0.053 mg/L (measured concentration) after 34 days test duration. In river water the NOEC for the most sensitive parameters hatchability and mean weight of larvae was 0.23 mg/L after 33 days test duration. The river water (Town River) had the following characteristics: pH = 6.4 - 6.9, total hardness = 62 mg/L CaCO3, 9.4 mg/L suspended solids, 0.59 mg/l MBAS and triethyleneglycol was used as carrier solvent. The well water had a hardness

of 28 -31 mg/L CaCO3, pH = 6.8-7.6 and isopropanol was used as carrier solvent. In well water the measured concentrations were equal to the nominal concentrations whereas in river water measured concentrations averaged 45-67% of the nominal concentrations.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

0.23 mg/L