sec-butylamine

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1976

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1976

Report date:

1976

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

other:

Version / remarks:

before the publication of the guideline

Principles of method if other than guideline:

One half ml of the sample was applied to one contact and one abraded skin site under standard gauze patches on each of three albino rabbits. The entire hair clipped truck of each animal was then covered with an impervious sleeve for a period of 24 hours.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

sec-butylamine

EC Number:

237-732-7

EC Name:

sec-butylamine

Cas Number:

13952-84-6

Molecular formula:

C4H11N

IUPAC Name:

butan-2-amine

Test animals

Species:

rabbit

Strain:

other: albino

Test system

Type of coverage:

occlusive

Preparation of test site:

other: applied on one intact and one abraded skin on each of three rabbits

Amount / concentration applied:

one half mL

Duration of treatment / exposure:

24 hours

Observation period:

48 hours

Number of animals:

3 rabbits

Details on study design:

One half ml of the sample was applied to one contact and one abraded skin site under standard gauze patches on each of three albino rabbits. The entire hair clipped truck of each animal was then covered with an impervious sleeve for a period of 24 hours.

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

edema score

Basis:

animal #1

Time point:

other: 15 minutes

Score:

4

Reversibility:

not reversible

Remarks on result:

other: The skin turned black within 15 minutes.24 hours after initial contact all sites consisted of large, round, black necrotic lesions.

Results 2

Irritation parameter:

edema score

Basis:

animal #2

Time point:

other: 15 minutes

Score:

4

Reversibility:

not reversible

Remarks on result:

other: The skin turned black within 15 minutes.24 hours after initial contact all sites consisted of large, round, black necrotic lesions.

Results 3

Irritation parameter:

edema score

Time point:

other: 15 minutes

Score:

4

Reversibility:

not reversible

Remarks on result:

other: The skin turned black within 15 minutes. 24 hours after initial contact all sites consisted of large, round, black necrotic lesions.

Applicant's summary and conclusion

Interpretation of results:

Category 1A (corrosive) based on GHS criteria

Executive summary:

One half of the sample was applied to one intact and one abraded skin site under standard gauze patches on each of three albino rabbits. The entire hair-clipped trunk of each animal was then covered with impervious sleeve fora period of 24 hours. The sample spread well beyond the intended sites of contact and caused pain in every instance. The skin turned black within 15 minutes.Twenty-four hours after initial contact all trzated sites consisted of large (5cm) round, black, necrotic lesions. These became dry, hard, and concave within 48 hours. Subsequently they began to crack and peel, exposing raw tissues.