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Reaction mass of 1,5-Naphthalenedisulfonic acid, 3,3'-[(4-methyl-1,2-phenylene)bis[imino(6-chloro-1,3,5-triazine-4,2-diyl)imino[2-(acetylamino)-5-methoxy-4,1-phenylene]-2,1-diazenediyl]]bis-, sodium salt (1:4) and 1,5-Naphthalenedisulfonic acid, 3,3'-[(3-methyl-1,2-phenylene)bis[imino(6-chloro-1,3,5-triazine-4,2-diyl)imino[2-(acetylamino)-5-methoxy-4,1-phenylene]-2,1-diazenediyl]]bis-, sodium salt (1:4)
EC number: 947-857-5 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Some information in this page has been claimed confidential.
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From October 09, 1998 to October 21, 1998
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 998
- Report date:
- 1998
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- (modified for coloured test substance)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Test material form:
- solid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Duplicate samples were taken at the start of the test from the freshly prepared test media (without algae) of all the concentrations and from the control.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The test medium of the highest test concentration of nominal 100 mg/L was prepared dissolving 100 mg of test substance completely in 1000 mL test water by stirring for 15 minutes.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Scenedesmus subspicatus
- Strain: CHODAT (86.81 SAG)
- Source (laboratory, culture collection): Sammlug von algenkuturen, pfanzenphysiologisches insstitutder, Universitat Gottingen.
- Age of inoculum (at test initiation): 3 d
- Method of cultivation: according to test guideline.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- 24 mg/L CaCO3
- Test temperature:
- 23˚C
- pH:
- 8 to 8.9
- Nominal and measured concentrations:
- Nominal concentrations: 100, 32, 10, 3.2, 1.0 and 0.32 mg/L
- Details on test conditions:
- The test was started (0 h) by inoculum of a biomass of 10,000 algal cells per mL test medium. These cells were taken from an exponentially growing pre culture, which was set up about 3 d prior to the test at the same conditions as the test.
The performed in Erlenmeyer flasks (50mL), each with 15 mL algal suspension continuously stirred by magnetic stirrers, 3 flasks per test concentrations and 6 flasks in the control. Each Erlenmeyer flask was placed in black cylinder, coasted inside with ammonium foil. The cylinders were covered with glass dishes, the dishes were covered with watch glass dishes to prevent evaporation. - Reference substance (positive control):
- not specified
Results and discussion
Effect concentrations
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
Any other information on results incl. tables
Results:
Experiment part A | ||
Parameter (0-72 h) | Biomass (mg/L) | Growth rate (mg/L) |
EC50 (95% conf limits) | >100 | >100 |
EC10 (95% conf limits) | 3.4 (0.1 -11) | 18 (4.7 -57) |
Experiment part B | ||
Parameter (0-72 h) | Biomass (mg/L) | Growth rate (mg/L) |
EC50 (95% conf limits) | >100 | >100 |
EC10 (95% conf limits) | 7.4 (2 -15) | 30 (15 -63) |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The modified test demonstrated that the observed algal growth inhibition had an indirect cause, namely the light filter effect of the coloured test substance. Toxic effects of the test substance could be excluded up to the highest concentration of 100 mg/L. The ErC50 was therefore > 100 mg/L. The substance was neither found acutely nor chronically toxic to freshwater green algae.
- Executive summary:
A study was conducted to determine the toxicity of the test substance to freshwater algae according to a modified version of OECD Guideline 201, in compliance with GLP. Desmodesmus subspicatus cells were exposed for 72 h under static conditions at concentrations of 100, 32, 10, 3.2, 1.0 and 0.32 mg/L. All test media were slightly to strongly coloured by the test substance. Analytical dose verification was conducted using spectrophotometry. The mean measured concentrations in the analysed test media varied from 94 to 98% of nominal values. Therefore, all biological results were related to nominal concentrations. The same inhibition of Desmodesmus subspicatus was observed when the algae grew in test water without test substance, but under reduced light intensities by the filter effect of the coloured test media as in the second parallel experimental part, where the algae grew in the test media was dissolved test substance. Thus, the modified test demonstrated that the observed algal growth inhibition had an indirect cause, namely the light filter effect of the coloured test substance. Toxic effects of the test substance could be excluded up to the highest concentration of 100 mg/L. The ErC50 was therefore > 100 mg/L (nominal) (Peither, 1998).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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